Analysis of prophages harbored by the human-adapted subpopulation of Staphylococcus aureus CC398.
Analysis of prophages harbored by the human-adapted subpopulation of Staphylococcus aureus CC398.
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DOI：
10.1016/j.meegid.2013.06.009
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发表时间：
2013-08
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影响因子：
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通讯作者：
N. van der Mee-Marquet;A. Corvaglia;A. Valentin;David Hernández;X. Bertrand;M. Girard;J. Kluytmans;P. Donnio;R. Quentin;P. François
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作者：
N. van der Mee-Marquet;A. Corvaglia;A. Valentin;David Hernández;X. Bertrand;M. Girard;J. Kluytmans;P. Donnio;R. Quentin;P. François
金黄色葡萄球菌克隆复合体 398 是一种与牲畜相关的病原体，因其定植和感染人类和动物的能力而构成全球性威胁。我们使用高分辨率全基因组微阵列、原噬菌体分析、免疫逃避簇表征和全基因组测序来研究原噬菌体在新兴的人类适应 CC398 亚群中的作用，该亚群与生活在无动物环境中的人类的侵袭性感染有关。我们对属于新兴亚群的 CC398 分离株特异携带的一种噬菌体和两种原噬菌体进行了表征。我们将噬菌体引入允许的无原噬菌体分离株中。我们研究了溶原性对宿主抵抗进一步噬菌体感染和转化、获得侵入人类细胞的能力以及表达原噬菌体编码的毒力因子的能力的影响。我们报告了有缺陷的 phiMR11 样辅助噬菌体的证据，名为 StauST398-5pro，与新兴的非 LA CC398 亚群特别相关。 StauST398-5pro 提供实质性保护，防止水平遗传转移至宿主。它与人类相关的β-转化原噬菌体相互作用，编码免疫调节蛋白，从而使毒力基因在应激情况下表达。我们的研究结果深入了解了噬菌体在毒力表达和新宿主适应性遗传信息传播中的作用。金黄色葡萄球菌分离株。我们证明，功能性原噬菌体元件可以调节宿主特异性，并为新兴的细菌种内克隆赋予新的毒力特征。
Staphylococcus aureusclonal complex 398 is a livestock-associated pathogen that poses a worldwide threat because of its ability to colonize and infect both humans and animals. We used high-resolution whole-genome microarrays, prophage profiling, immune evasion cluster characterization and whole-genome sequencing to investigate the roles of prophages in the emerging human-adapted subpopulation of CC398 that has been associated with invasive infections in humans living in animal-free environments.We characterized one phage and two prophages specifically harbored by CC398 isolates belonging to the emerging subpopulation. We introduced the phage into permissive prophage-free isolates. We investigated the effects of lysogeny on the host ability to resist further phage infection and transformation, to acquire the capacity to invade human cells, and to express virulence factors encoded by prophages. We report evidence of a defective ϕMR11-like helper prophage, named StauST398-5pro, specifically associated with the emerging non-LA CC398 subpopulation. StauST398-5pro confers substantial protection against horizontal genetic transfer to its host. It interacts with a human-associated β-converting prophage encoding immune-modulating proteins such that virulence genes are expressed during stress situations.Our findings provide insight into the role of phages in the expression of virulence and in the spread of genetic information among new host-adaptedS. aureusisolates. We demonstrate that functional prophage elements can condition host specificity and confer new virulence traits on emerging intra-species clones of bacteria.