Probing the interactions of CdTe quantum dots with pseudorabies virus.
Probing the interactions of CdTe quantum dots with pseudorabies virus.
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探讨 CdTe 量子点与伪狂犬病病毒的相互作用
DOI：
10.1038/srep16403
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发表时间：
2015-11-10
影响因子：
4.6
通讯作者：
Xiao S
中科院分区：
文献类型：
--
作者：
Du T;Cai K;Han H;Fang L;Liang J;Xiao S
量子点（QD）已经成为最有前途的荧光材料之一，用于跟踪活细胞中的病毒感染。然而，关于量子点如何与病毒相互作用的几个问题仍然没有解决。本文以伪狂犬病病毒为模型，研究了谷胱甘肽（GSH）修饰的CdTe量子点对病毒的作用。一步生长曲线和荧光共定位分析表明，CdTe量子点通过抑制PRV的入侵，在PRV复制周期的早期阶段抑制PRV的增殖，但对PRV的穿透能力无明显影响。荧光光谱分析表明，PRV加入后30 min内，量子点的尺寸逐渐减小。在量子点与PRV相互作用过程中检测到Cd 2+的释放，导致可以感染细胞的病毒数量减少。进一步的拉曼光谱和圆二色性（CD）光谱分析表明，吸附在病毒表面的CdTe量子点改变了病毒表面蛋白的结构，从而抑制了病毒的复制。该研究有助于深入了解病毒的致病机制，并为量子点标记病毒的研究提供了基础。
Quantum dots (QDs) have become one of the most promising luminescent materials for tracking viral infection in living cells. However, several issues regarding how QDs interact with the virus remain unresolved. Herein, the effects of Glutathione (GSH) capped CdTe QDs on virus were investigated by using pseudorabies virus (PRV) as a model. One-step growth curve and fluorescence colocalization analyses indicate that CdTe QDs inhibit PRV multiplication in the early stage of virus replication cycle by suppressing the invasion, but have no significant effect on the PRV penetration. Fluorescence spectrum analysis indicates that the size of QDs is reduced gradually after the addition of PRV within 30 min. Release of Cd2+was detected during the interaction of QDs and PRV, resulting in a decreased number of viruses which can infect cells. Further Raman spectra and Circular Dichroism (CD) spectroscopy analyses reveal that the structure of viral surface proteins is altered by CdTe QDs adsorbed on the virus surface, leading to the inhibition of virus replication. This study facilitates an in-depth understanding of the pathogenic mechanism of viruses and provides a basis for QDs-labeled virus research.