Visualization of proteins in acrylamide gels using ultraviolet illumination
Visualization of proteins in acrylamide gels using ultraviolet illumination
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DOI：
10.1006/abio.2001.5488
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发表时间：
2002-02-01
影响因子：
2.9
通讯作者：
Starkey, J
中科院分区：
文献类型：
--
作者：
Kazmin, D;Edwards, RA;Starkey, J
聚丙烯酰胺凝胶中的蛋白质可以通过在三氯乙酸或氯仿中浸泡，然后在三氯化合物存在下进行色氨酸的UV反应来快速可视化，产生在可见光区充分发射的产物，以识别凝胶上蛋白质条带的位置。这种方法可以用来快速识别蛋白质条带的凝胶在不到20分钟。在薄的聚丙烯酰胺凝胶，1.0马克杯的蛋白质可以很容易地检测到典型的色氨酸百分比的蛋白质。(C)2002年，Elsevier Science。
Proteins in polyacrylamide gels can be rapidly visualized by soaking in trichloroacetic acid or chloroform followed by UV reaction of tryptophan in the presence of trichlorocompounds yields products that emit sufficiently in the visible region to identify the location of the protein bands on the gel. This method can be used to rapidly identify protein bands on a gel in less than 20 min. On thin polyacrylamide gels, 1.0 mug of protein can easily be detected for proteins with typical tryptophan percentages. (C) 2002 Elsevier Science.