Characterization of regulatory T cells in decidua of miscarriage cases with abnormal or normal fetal chromosomal content
Characterization of regulatory T cells in decidua of miscarriage cases with abnormal or normal fetal chromosomal content
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DOI：
10.1016/j.jri.2012.12.001
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发表时间：
2013-03-01
影响因子：
3.4
通讯作者：
Saito, Shigeru
中科院分区：
文献类型：
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作者：
Inada, Kumiko;Shima, Tomoko;Saito, Shigeru
据报道，在反复妊娠丢失的病例中，调节性T(Treg)细胞减少。为了了解Treg细胞在人类妊娠中的作用，我们研究了Treg细胞在蜕膜中的频率、定位和特征。胚胎核型正常流产组(n=10)蜕膜CD3(+)、CD8(-)细胞中Foxp3(+)细胞比例明显低于正常妊娠组(n=10)。然而，在胚胎核型异常的流产中，这些频率与正常妊娠相似。接下来，我们用流式细胞术研究了Treg细胞增殖标记Ki67和功能标记CCR5的表达。Foxp3(+)CD4(+)T细胞在正常妊娠流产组(n=10)明显低于正常妊娠组(n=15)和异常流产组(n=14)。正常妊娠流产组Ki67(-)Foxp3(+)CD4(+)T细胞显著低于正常妊娠组(P<0.05)。而Ki67(+)Foxp3(+)CD4(+)细胞和CCR5(+)Foxp3(+)CD4(+)细胞在三组间差异无统计学意义。这些结果提示，蜕膜中Ki67(-)Treg细胞的增加可能在免疫耐受的诱导中起重要作用，而免疫药物性妊娠丢失可能是由于植入部位Ki67(-)Treg细胞减少所致。(C)2012爱思唯尔爱尔兰有限公司。保留所有权利。
Decreased regulatory T (Treg) cells have been reported in cases of recurrent pregnancy loss. To understand the role of Treg cells in human pregnancy, we have studied the frequency, localization and characterization of Treg cells in the decidua. The frequency of Foxp3(+) cells among CD3(+)CD8(-) cells at the decidua basalis in cases of miscarriage with a normal embryo karyotype (n = 10) was significantly lower than in normally progressing pregnancies (n = 10). However, those frequencies in miscarriage with an abnormal embryo karyotype were similar to normally progressing pregnancies. Next, we used flow cytometry to study Treg cell expression of the proliferation marker Ki67 and functional Treg marker CCR5. The frequency of Foxp3(+)CD4(+) T cells in miscarriage with a normal embryo (n = 10) was significantly lower than those in normally progressing pregnancies (n = 15) and in miscarriage with an abnormal embryo (n = 14). In miscarriage with a normal embryo, the population of Ki67(-)Foxp3(+)CD4(+) T cells was significantly smaller than in normal pregnancy. However, the frequencies of Ki67(+)Foxp3(+)CD4(+) cells and CCR5(+)Foxp3(+)CD4(+) cells were not different between the three groups. These data suggest that increased Ki67(-) Treg cells in the decidua basalis may play an important role in the induction of immune tolerance, and that immune-medicated pregnancy loss may be caused by decreased Ki67(-) Treg cells in the implantation site. (C) 2012 Elsevier Ireland Ltd. All rights reserved.