ARACHIDONIC ACID METABOLISM IN MURINE FIBROSARCOMA CELLS WITH DIFFERING IN VIVO AND IN VITRO CHARACTERISTICS
ARACHIDONIC ACID METABOLISM IN MURINE FIBROSARCOMA CELLS WITH DIFFERING IN VIVO AND IN VITRO CHARACTERISTICS
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具有不同体内外特征的鼠纤维肉瘤细胞中的花生四烯酸代谢
DOI：
10.1002/ijc.1985.36.3.383
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发表时间：
1985
影响因子：
6.4
通讯作者：
J. Varani
中科院分区：
文献类型：
--
作者：
S. Fligiel;P. Perone;J. Varani
花生四烯酸代谢进行了检查，在一系列强烈恶性小鼠纤维肉瘤细胞系和一系列弱恶性系分离自相同的肿瘤。在未刺激状态和用12 - 0-十四烷酰基佛波醇乙酸酯（TPA）、层粘连蛋白或纤连蛋白刺激后检查细胞。从先前的研究中可知，所有3种药物均可诱导小鼠纤维肉瘤细胞的粘附和运动。当细胞用3 H-花生四烯酸预标记时，所有3种试剂均刺激放射性释放至上清液中。对TPA的反应是快速的，而反应较慢，但持续时，无论是层粘连蛋白或纤连蛋白被用作刺激剂。这是令人感兴趣的，因为TPA在相同的细胞中诱导快速但短暂的粘附反应，而层粘连蛋白和纤连蛋白诱导缓慢、持续的反应。放射免疫分析法检测表明，对照细胞和刺激细胞都能产生多种脂氧合酶和环氧合酶代谢产物。在数量上，强恶性细胞比它们的弱恶性对应物更活跃。与相应的弱恶性细胞相比，它们向上清液中释放了更多的放射性，并产生了更多的花生四烯酸代谢产物，特别是前列腺素E2。这是令人感兴趣的，因为先前的研究表明，虽然强恶性细胞和弱恶性细胞在粘附测定中对TPA、层粘连蛋白和纤连蛋白都有反应，但只有强恶性细胞表现出定向运动性（趋化性和趋触性）。
Arachidonic acid metabolism was examined in a series of strongly malignant murine fibrosarcoma cell lines and in a series of weakly malignant lines isolated from the same tumors. The cells were examined in the unstimulated state and after stimulation with 12‐0‐tetradecanoyl phorbol acetate (TPA), laminin or fibronectin . All 3 agents were known from previous studies to induce adherence and motility in the murine fibrosarcoma cells. When the cells were prelabelled with 3H‐arachidonic acid, all 3 agents stimulated the release of radioactivity into the supernatant fluids. The response to TPA was rapid while the response was slower but sustained when either laminin or fibronectin was used as the stimulating agent. This is of interest because TPA induces a rapid but transient adherence response in the same cells while laminin and fibronectin induce a slow, sustained response. Examination by radioimmunoassay procedures indicated that both control cells and stimulated cells were able to produce a variety of lipoxygenase and cyclooxygenase metabolites. In quantitative terms, the strongly Malignant cells were more active than their weakly malignant counterparts. They released greater amounts of radioactivity into the supernatant fluid and produced a greater quantity of arachidonic acid Metabolites, particularly prostaglandin E2, than did the corresponding weakly malignant cells. This is of interest because previous studies have shown that while both the strongly and weakly malignant cells respond in the adherence assay to TPA, laminin and fibronectin, only the strongly malignant cells demonstrate directional motility (chemotaxis and haptetaxis).
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影响因子：
11.2
作者：
H. Koeffler;M. Bar‐eli;M. Territo
通讯作者：
H. Koeffler;M. Bar‐eli;M. Territo
12-O-tetradecanoylphorbol-13-acetate 刺激 3T3 细胞的 TPA 非增殖变体释放花生四烯酸、前列腺素 E2 和前列腺素 F2 α。
DOI：
10.1016/s0006-291x(83)80135-1
发表时间：
1983
影响因子：
3.1
作者：
Butler-Gralla,E;Taplitz,S;Herschman,HR
通讯作者：
Herschman,HR
前列腺素 E1 的抗炎作用：甲酰肽趋化受体对大鼠中性粒细胞的体内调节。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fantone,JC;Marasco,WA;Elgas,LJ;Ward,PA
通讯作者：
Ward,PA
佛波醇肉豆蔻酸酯乙酸酯诱导 Walker 256 癌肉瘤细胞的粘附。
影响因子：
11.2
作者：
Varani,J;Fantone,JC
通讯作者：
Fantone,JC
不同恶性肿瘤的两种纤维肉瘤亚群的趋化反应性的比较。
DOI：
--
发表时间：
1981
期刊：
The American journal of pathology
影响因子：
--
作者：
Orr,FW;Varani,J;Delikatny,J;Jain,N;Ward,PA
通讯作者：
Ward,PA