Chemokine-dependent signaling pathways in the peripheral nervous system.
Chemokine-dependent signaling pathways in the peripheral nervous system.
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DOI：
10.1007/978-1-62703-426-5_2
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发表时间：
2013-01-01
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影响因子：
--
通讯作者：
Ubogu, Eroboghene E
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文献类型：
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作者：
Ubogu, Eroboghene E
趋化因子及其G蛋白偶联受体在发育、稳态以及先天性和适应性免疫应答中起重要作用。外周神经系统中的病理性趋化因子信号传导途径可以使用血-神经屏障（BNB）的人体外模型和称为严重鼠实验性自身免疫性神经炎（EAN）的急性外周神经炎症的可靠模型在外周神经中进行研究。本章描述了一种流量依赖性人白细胞BNB运输试验和可靠的诱导EAN在雌性SJL/J小鼠作为工具，研究促炎趋化因子依赖性信号在外周神经。
Chemokines and their G-protein-coupled receptors play important roles in development, homeostasis, and the innate and adaptive immune response. Pathologic chemokine signaling pathways in the peripheral nervous system can be studied in peripheral nerves using human in vitro models of the blood-nerve barrier (BNB) and a reliable model of acute peripheral nerve inflammation called severe murine experimental autoimmune neuritis (EAN). This chapter describes a flow-dependent human leukocyte-BNB trafficking assay and the reliable induction of EAN in female SJL/J mice as tools to study pro-inflammatory chemokine-dependent signaling in peripheral nerves.