Local raster scanning for high-speed imaging of biopolymers in atomic force microscopy
Local raster scanning for high-speed imaging of biopolymers in atomic force microscopy
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DOI：
10.1063/1.3600558
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发表时间：
2011-06-01
影响因子：
1.6
通讯作者：
Andersson, Sean B.
中科院分区：
文献类型：
--
作者：
Chang, Peter I.;Huang, Peng;Andersson, Sean B.
一种新的算法描述和说明了高速成像的生物聚合物和其他stringlike样品使用原子力显微镜。该方法使用实时测量来操纵仪器的尖端，以定位感兴趣的样品上的扫描区域。根据样品的不同，扫描时间可以减少一个数量级或更多，同时保持图像分辨率。图像是通过使用修改的克里格算法插值的非光栅数据。该方法被证明使用物理模拟，包括致动器和悬臂梁动力学，非线性尖端-样品相互作用，和测量噪声，以及通过扫描实验，其中两个轴的纳米定位阶段是由该算法使用模拟的高度数据转向。(C)2011年美国物理学会。[doi：10.1063/1.3600558]
A novel algorithm is described and illustrated for high speed imaging of biopolymers and other stringlike samples using atomic force microscopy. The method uses the measurements in real-time to steer the tip of the instrument to localize the scanning area over the sample of interest. Depending on the sample, the scan time can be reduced by an order of magnitude or more while maintaining image resolution. Images are generated by interpolating the non-raster data using a modified Kriging algorithm. The method is demonstrated using physical simulations that include actuator and cantilever dynamics, nonlinear tip-sample interactions, and measurement noise as well as through scanning experiments in which a two-axis nanopositioning stage is steered by the algorithm using simulated height data. (C) 2011 American Institute of Physics. [doi:10.1063/1.3600558]