Antioxidant activity and genotoxic effect on HeLa cells of phenolic compounds from infusions of Quercus resinosa leaves
Antioxidant activity and genotoxic effect on HeLa cells of phenolic compounds from infusions of Quercus resinosa leaves
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DOI：
10.1016/j.foodchem.2009.01.050
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发表时间：
2009-08-15
期刊：
影响因子：
8.8
通讯作者：
Azalia Lujan-Garcia, Blanca
中科院分区：
文献类型：
--
作者：
Elizabeth Rocha-Guzman, Nuria;Alberto Gallegos-Infante, Jose;Azalia Lujan-Garcia, Blanca
水灌注的成熟和新鲜的栎叶的抗氧化活性和对HeLa细胞的遗传毒性作用进行了评价。墨西哥土著人过去常喝Q。树脂使茶成为一种清爽的饮料。将风干的叶子粉碎并在水中煮沸，然后测定其酚类含量和缩合单宁。采用高效液相色谱法测定了栎叶浸液中15种酚类成分的色谱图。采用DPPH法和脱氧核糖法对银杏叶浸液提取物的抗氧化能力进行了体外研究。Q.通过单细胞电泳试验（彗星试验）在HeLa细胞上评估树脂叶提取物及其潜在机制。结果表明，新鲜的叶子输液增加了氧化过程和其他损伤的DNA在转化的人类细胞。从Q，resinosa鲜叶可能作为一个潜在的来源酚类化合物具有抗癌活性。(C)2009爱思唯尔有限公司保留所有权利。
Water infusions of mature and fresh Quercus resinosa leaves were evaluated for antioxidant activity and genotoxic effects on HeLa cells. Native Mexicans used to drink Q. resinoso leaves tea as a refreshing beverage. The air dried leaves were pulverised and boiled in water, then their phenolic content and condensed tannins were determined. The chromatographic profile of 15 phenolic components in Quercus leaves infusions was also determined by HPLC. In vitro analysis of antioxidant capacity of leaves infusion extracts were performed by the DPPH method and the deoxyribose assay. The genotoxicity of Q. resinosa leaves extracts was evaluated on HeLa cells as well as its underlying mechanism by the single-cell electrophoresis assay (comet assay). Results show that fresh leaves infusions increase the oxidative process and other damage to DNA in transformed human cells. Fresh leaves from Q, resinosa may serve as a potential source of phenolics with anticancer activity. (C) 2009 Elsevier Ltd. All rights reserved.