Identification of F-box proteins that are involved in resistance to methylmercury in Saccharomyces cerevisiae
Identification of F-box proteins that are involved in resistance to methylmercury in Saccharomyces cerevisiae
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DOI：
10.1016/j.febslet.2006.11.045
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发表时间：
2006-12-22
期刊：
影响因子：
3.5
通讯作者：
Naganuma, Akira
中科院分区：
文献类型：
--
作者：
Hwang, Gi-Wook;Ishida, Yosuke;Naganuma, Akira
我们寻找可能与保护酿酒酵母免受甲基汞毒性作用的机制有关的 F-box 蛋白。我们发现 Hrt3 和 YIr224w 的过度表达使酵母细胞对甲基汞具有抗性。在蛋白酶体抑制剂存在的情况下，过表达 Hrt3 和 Ylr224w 的酵母细胞对甲基汞几乎没有抵抗力。我们的结果表明，酵母细胞中存在一些增强甲基汞毒性的蛋白质，而且，Hrt3 或 YIr224w 的过度表达可以通过增强该蛋白质的多泛素化及其在蛋白酶体中的降解来赋予对甲基汞的抗性。 (c) 2006 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
We searched for F-box proteins that might be related to the mechanism that protects Saccharomyces cererisiae against the toxic effects of methylmercury. We found that overexpression of Hrt3 and of YIr224w rendered yeast cells resistant to methylmercury. Yeast cells that overexpressed Hrt3 and Ylr224w were barely resistant to methylmercury in the presence of a proteasome inhibitor. Our results suggest the existence of some protein(s) that enhances the toxicity of methylmercury in yeast cells and, also, that overexpression of Hrt3 or YIr224w can confer resistance to methylmercury by enhancing the polyubiquitination of this protein(s) and its degradation in proteasomes. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.