Effects of melittin on adipocyte metabolism unrelated to lysophospholipid accumulation.
Effects of melittin on adipocyte metabolism unrelated to lysophospholipid accumulation.
复制标题
蜂毒肽对与溶血磷脂积累无关的脂肪细胞代谢的影响。
DOI：
10.1016/0304-4165(81)90096-9
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Li,SY
中科院分区：
文献类型：
--
作者：
Fain,JN;Kabnick,KS;Li,SY
蜂毒素加入大鼠或仓鼠脂肪细胞中，可抑制脂肪分解、环磷酸腺苷蓄积和葡萄糖氧化。低浓度蜂毒素在刺激葡萄糖代谢或抑制脂解方面不是胰岛素样的。较高浓度的蜂毒素可溶解脂肪细胞。在蜂毒素存在下，细胞磷脂被释放到介质中并被水解，溶血磷脂几乎没有积累。只有在被磷脂酶A2污染的蜂毒素孵育的脂肪细胞中，才能看到溶血磷脂的明显积累，而且这是在培养液中。这些数据表明，蜂毒素对脂肪细胞的毒性作用不是由于溶血磷脂的积累，而是由于膜磷脂的损失或膜蛋白的改变。
Melittin addition to rat or hamster adipocytes resulted in inhibition of lipolysis, cyclic AMP accumulation and glucose oxidation. Low concentrations of melittin were not insulin-like with respect to either stimulation of glucose metabolism or inhibition of lipolysis. Higher concentrations of melittin lysed adipocytes. In the presence of melittin, cellular phospholipids were released to the medium and hydrolyzed with little accumulation of lysophospholipids. Only in adipocytes incubated with melittin contaminated with phospholipase A2was any appreciable accumulation of lysophospholipids seen and this was in the medium. These data suggest that the toxic effects of melittin on adipocytes are not due to the accumulation of lysophospholipids but rather to the loss of membrane phospholipids or alterations in membrane proteins.