Alternative initiation of translation and time-specific phosphorylation yield multiple forms of the essential clock protein FREQUENCY
Alternative initiation of translation and time-specific phosphorylation yield multiple forms of the essential clock protein FREQUENCY
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DOI：
10.1016/s0092-8674(00)80227-5
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发表时间：
1997-05-02
期刊：
影响因子：
64.5
通讯作者：
Dunlap, JC
中科院分区：
文献类型：
--
作者：
Garceau, NY;Liu, Y;Dunlap, JC
频率(FRQ)基因编码基于转录/翻译的负反馈环的中心成分，组成脉孢菌昼夜节律振荡器的核心；与FRQ相关的转录后调控令人惊讶地复杂。翻译起始点的替代使用产生了两种形式的FRQ，其水平在FRQ转录高峰期后4-6小时达到峰值。每种形式的FRQ在一天的过程中逐渐被磷酸化，从而提供一些在时间上不同的FRQ产品。这些调控过程的动力学表明，在时钟的观点中，相对快速的事件涉及FRQ合成的翻译调控和FRQ对转录水平的负反馈，随后是较慢的翻译后调控，最终推动FRQ的周转和FRQ基因的重新激活。
The frequency (frq) gene encodes central components of the transcription/translation-based negative-feedback loop comprising the core of the Neurospora circadian oscillator; posttranscriptional regulation associated with FRQ is surprisingly complex. Alternative use of translation initiation sites gives rise to two forms of FRQ whose levels peak 4-6 hr following the peak of frq transcript. Each form of FRQ is progressively phosphorylated over the course of the day, thus providing a number of temporally distinct FRQ products. The kinetics of these regulatory processes suggest a view of the clock where relatively rapid events involving translational regulation in the synthesis of FRQ and negative feedback of FRQ on fro transcript levels are followed by slower posttranslational regulation, ultimately driving the turnover of FRQ and reactivation of the frq gene.