GRIDLOCK, A LOCALIZED HERITABLE VASCULAR PATTERNING DEFECT IN THE ZEBRAFISH
GRIDLOCK, A LOCALIZED HERITABLE VASCULAR PATTERNING DEFECT IN THE ZEBRAFISH
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DOI：
10.1038/nm1195-1143
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发表时间：
1995-11-01
期刊：
影响因子：
82.9
通讯作者：
FISHMAN, MC
中科院分区：
文献类型：
--
作者：
WEINSTEIN, BM;STEMPLE, DL;FISHMAN, MC
我们正在使用斑马鱼，Danio rerio，来鉴定产生和形成脊椎动物脉管系统的基因。我们已经分离出一种隐性突变，gridlock（m145）（grl（m145）），其中流向尾部的血液受到局部血管缺陷的阻碍。使用一种新的微血管造影方法，我们表明，封锁是在前干，在那里的成对的外侧背侧腹主动脉通常合并，形成单一的中线主动脉。在大多数突变的胚胎中，动脉-静脉分流和侧支血管发育，绕过病变并重建尾部血流。grl缺陷类似于主动脉缩窄，一种病因不明的人类先天性心血管畸形，在病变的位置和后果以及突变体对侧支血管的依赖性方面。
We are using the zebrafish, Danio rerio, to identify genes that generate and pattern the vertebrate vasculature. We have isolated a recessive mutation, gridlock(m145) (grl(m145)) in which blood flow to the tail is impeded by a localized vascular defect. Using a novel microangiographic method, we show that the blockade is in the anterior trunk, where the paired lateral dorsal aortae normally merge to form the single midline aorta. Arterial-venous shunts and collateral vessels develop in most mutant embryos, bypassing the lesion and reconstituting caudal blood flow. The grl defect resembles coarctation of the aorta, a human congenital cardiovascular malformation of unknown aetiology, in the location of the lesion and its consequences and in the mutants' dependence on collateral vessels for survival.