Studies on the chemistry of arterial wall, XVII. Metabolic characteristics of different types of chondroitin sulfate-dermatan sulfate hybrids in arterial tissue.
Studies on the chemistry of arterial wall, XVII. Metabolic characteristics of different types of chondroitin sulfate-dermatan sulfate hybrids in arterial tissue.
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动脉壁化学研究，XVII。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
E. Buddecke
中科院分区：
文献类型：
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作者：
K. Figura;W. Kiowski;E. Buddecke
1)牛动脉组织中的硫酸软骨素和硫酸皮肤素以共聚物的形式存在，两者之间存在不同程度的杂交。通过对动脉组织CS-DS制剂的进一步分离，可以分离出富含皮肤素和20%硫酸软骨素的组分(称为富含DS的杂交物)和富含17%DS的硫酸软骨素作为共聚组分(富含CS的杂交物)。2)当动脉组织段与[14C]氨基葡萄糖预先孵育时，糖胺多聚糖中95%的放射性存在于所有CS-DS亚组分的氨基半乳糖中，而与[14C]葡萄糖预先孵育后，14C放射性与氨基半乳糖和糖醛酸的相对比例为51：49。在这两个实验中，富含DS杂交种的比放射性是富含CS杂交种的两倍。3)软骨素AC和ABC裂解酶对杂化CS-DS亚组分的酶促降解表明，富含DS的杂交种释放的CS和DS双糖单元的比放射性是富含CS的杂交种的两倍，但在每个杂化组分中，CS和DS单元的半乳糖胺部分及其葡萄糖和艾糖醛酸组分表现出相同的比放射性。4)这些结果有力地支持了这样的假设，即形成具有不同比例CS和DS的杂化CS-DS蛋白多糖时存在不同的隔室。
1) Chondroitin sulfate and dermatan sulfate of bovine arterial tissue exist as copolymers with a varying degree of hybridization between chondroitin and dermatan sulfates. A fraction rich in dermatan sulfate hybridized with 20% chondroitin sulfate (termed DS-rich hybrid) and a fraction rich in chondroitin sulfate containing 17% DS as copolymer constituent (CS-rich hybrid) can be isolated by the subfractionation of the arterial tissue CS-DS preparation. 2) When arterial tissue segments were preincubated with [14C]glucosamine, 95% of the radioactivity incorporated into the glycosaminoglycans was found to be present in the galactosamine moiety of all of the CS-DS subfractions, whereas the relative proportion of 14C radioactivity incorporated into the galactosamine and uronic acid components was 51:49 following preincubation with [14C]glucose. In both experiments the specific radioactivity of the DS-rich hybrids was twice as high as that of the CS-rich hybrids. 3) Enzymatic degradation of the hybrid CS-DS subfractions by chondroitin AC and ABC lyases revealed that the specific radioactivity of the CS and DS disaccharide units released from the DS-rich hybrids was twice as high as those isolated from the CS-rich hybrids, but within each hybrid fraction the galactosamine moieties of the CS and DS units and their glucuronic and iduronic acid components exhibited equal specific radioactivities. 4) The results strongly support the assumption that distinct compartments exist for the formation of hybrid CS-DS proteoglycans with different proportions of CS and DS.