Kinetics of double strand break repair in the DNA of X-irradiated synchronized mammalian cells.
Kinetics of double strand break repair in the DNA of X-irradiated synchronized mammalian cells.
复制标题
X 射线照射的同步哺乳动物细胞 DNA 中双链断裂修复的动力学。
DOI：
10.1080/09553008314550681
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
P. Bryant
中科院分区：
文献类型：
--
作者：
D. Blöcher;M. Nüsse;P. Bryant
2. 材料和方法使用了埃利希腹水肿瘤细胞系（Karzel 1965），其常规培养方法已在之前描述过（Iliakis and Pohlit 1979）。细胞在悬浮中生长，每日传代，从8 × 10.5个细胞/ml到2 × 105个细胞/mi。翻倍时间为12小时。细胞同步的方法包括选择gi细胞在一个区域转子离心，随后胸苷阻断。有关这种方法和同步程度的详细信息，请参见Nusse（1982）。该方法产生在G1/ s边界捕获的细胞，然后将其在新鲜生长培养基中洗涤，并允许继续到现在的地址：Klinikum der Johann Wolfgang Goethe Universitat， Gustav Embden生物化学中心，7 Theodor Stern Kai, Frankfurt am Main, FR Germany。
2. Materials and methods A line of Ehrlich ascites tumour cells (Karzel 1965) was used, the methods of routine culture of which have been described previously (Iliakis and Pohlit 1979). Cells grew in suspension and were maintained by daily passage from 8 x 10 5 cells/ml to 2 x 105cells/mi. The doubling time was 12 hours. Cells were synchronized by a method involving selection of GI-cells by centrifugation in a zonal rotor, and subsequent thymidine block. For details of this method and the degree of synchronization, see Nusse (1982). The method yielded cells arrested at the G1/S-border, which were then washed in fresh growth medium and allowed to proceed t Present address: Klinikum der Johann Wolfgang Goethe Universitat, Gustav Embden Zentrum der Biologischen Chemie, 7 Theodor Stern Kai, Frankfurt am Main, FR Germany.