A novel adjuvant for vaccine development in the aged
A novel adjuvant for vaccine development in the aged
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DOI：
10.1016/j.vaccine.2010.10.008
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发表时间：
2010-12-06
期刊：
影响因子：
5.5
通讯作者：
Phillips, Joy A.
中科院分区：
文献类型：
--
作者：
Morgan, Edward L.;Thoman, Marilyn L.;Phillips, Joy A.
人C5 a（65-74）（EP 67）的构象偏向性、反应选择性激动剂在体外激活来自老龄C57 B1/6小鼠的抗原呈递细胞（APC），并在体内产生老龄小鼠中的抗原（Ag）特异性抗体（Ab）反应。EP 67诱导促炎细胞因子IL-6、TNF α和INF γ从从老年和年轻小鼠获得的脾APC释放。老年和年轻的小鼠产生高Ag特异性IgG抗体滴度时，免疫与含EP 67的疫苗卵清蛋白（OVA-EP 67）和蛋白质（rPrp 1）从球孢子菌细胞壁（rPrp 1-EP 67）。与用吸附于明矾的OVA（OVA/明矾）和与CpG混合的Prp 1（rPrp 1 +CpG）免疫的小鼠相比，用含有EP 67的疫苗免疫在年轻和老年小鼠中均导致更高的IgG滴度。用含EP 67的疫苗免疫的老年和年轻小鼠产生的IgG 1和IgG 2b滴度高于用OVA/明矾或Prp 1 +CpG免疫的年龄匹配的小鼠。这些结果表明，EP 67在老年小鼠中诱导的体液免疫不能用明矾和CpG获得。这些结果支持使用EP 67作为适合老年人的潜在疫苗佐剂。(C)2010爱思唯尔有限公司版权所有。
A conformationally-biased, response-selective agonist of human C5a(65-74) (EP67) activated antigen presenting cells (APC) from aged C57Bl/6 mice in vitro and the generation of antigen (Ag)-specific antibody (Ab) responses in aged mice in vivo. EP67, induced the release of the pro-inflammatory cytokines IL-6, TNF alpha, and INF gamma from splenic APCs obtained from both aged and young mice. Both aged and young mice produced high Ag-specific IgG Ab titers when immunized with EP67-containing vaccines to ovalbumin (OVA-EP67) and to a protein (rPrp1) from the cell wall of Coccidioides (rPrp1-EP67). Immunization with EP67-containing vaccines resulted in higher IgG titers in both young and aged mice compared to mice immunized with OVA adsorbed to alum (OVA/alum) and Prp1 admixed with CpG (rPrp1 +CpG). Aged and young mice immunized with the EP67-containing vaccines generated higher titers of IgG1 and IgG2b relative to their aged-matched counterparts immunized with OVA/alum or Prp1 +CpG. These results indicate that EP67 induces humoral immunity in aged mice not obtainable with alum and CpG. These results support the use of EP67 as a potential vaccine adjuvant suited to the elderly. (C) 2010 Elsevier Ltd. All rights reserved.