Gene expression and plant hormone levels in two contrasting rice genotypes responding to brown planthopper infestation.
Gene expression and plant hormone levels in two contrasting rice genotypes responding to brown planthopper infestation.
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DOI：
10.1186/s12870-017-1005-7
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发表时间：
2017-02-28
影响因子：
5.3
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Li C;Luo C;Zhou Z;Wang R;Ling F;Xiao L;Lin Y;Chen H
褐飞虱(BPH)是一种毁灭性的水稻刺吸性害虫，名为褐飞虱Nilparvata luens StóL。植物激素水杨酸(SA)和茉莉酸(JA)在植物-害虫相互作用中起着重要作用。许多分离的调节褐飞虱抗性的水稻基因参与了SA、JA和乙烯的代谢或信号通路。‘Rathu Heenati’(RH)是一种对所有褐飞虱生物型都具有高水平、广谱抗性的水稻品种。本研究以RH为研究材料，以对褐飞虱敏感的水稻品种‘台中原生1号’(TN1)为对照。基因芯片分析揭示了褐飞虱侵染下RH在基因组水平的抗性反应。还测定了褐飞虱侵染后RH和TN1幼苗中SA和JA的水平。表达谱聚类表明，1467个差异探针集可能与RH的结构性抗性相关，67个差异探针集可能与RH对褐飞虱侵染的抗性相关。维恩图分析显示192套RH特异性和BPH可诱导的探针组。最后，基于表达模式聚类和维恩图分析，筛选出23个褐飞虱抗性相关候选基因。在RH中，褐飞虱侵染后SA含量显著增加，JA含量显著降低，前者发生在后者之前。在RH中，SA途径中的差异基因是与合成相关的，并且在BPH侵染后上调。JA途径中的差异基因也表达上调。它们是茉莉酸ZIM结构域转录因子，是JA途径的重要负调控因子。相比之下，参与ET途径的基因在RH中受BPH侵染的影响较小。DNA序列分析表明，大多数BPH侵染诱导基因可能受遗传背景的反式调控，而不是它们的启动子。我们分析了褐飞虱侵染下RH和TN1的整体基因表达，以及SA和JA水平的变化。SA在水稻对褐飞虱的抗性反应中起主导作用。我们的研究结果将有助于了解RH对褐飞虱抗性的分子基础，并有助于鉴定新的抗性相关基因，为选育抗褐飞虱水稻品种奠定基础。本文的在线版本(doi：10.1186/s12870-0171005-7)包含补充材料，授权用户可以使用。
The brown planthopper (BPH; Nilaparvata lugens Stål) is a destructive piercing-sucking insect pest of rice. The plant hormones salicylic acid (SA) and jasmonic acid (JA) play important roles in plant–pest interactions. Many isolated rice genes that modulate BPH resistance are involved in the metabolism or signaling pathways of SA, JA and ethylene. ‘Rathu Heenati’ (RH) is a rice cultivar with a high-level, broad-spectrum resistance to all BPH biotypes. Here, RH was used as the research material, while a BPH-susceptible rice cultivar ‘Taichung Native 1’ (TN1) was the control. A cDNA microarray analysis illuminated the resistance response at the genome level of RH under BPH infestation. The levels of SA and JA in RH and TN1 seedlings after BPH infestation were also determined. The expression pattern clustering indicated that 1467 differential probe sets may be associated with constitutive resistance and 67 with the BPH infestation-responsive resistance of RH. A Venn diagram analysis revealed 192 RH-specific and BPH-inducible probe sets. Finally, 23 BPH resistance-related gene candidates were selected based on the expression pattern clustering and Venn diagram analysis. In RH, the SA content significantly increased and the JA content significantly decreased after BPH infestation, with the former occurring prior to the latter. In RH, the differential genes in the SA pathway were synthesis-related and were up-regulated after BPH infestation. The differential genes in the JA pathway were also up-regulated. They were jasmonate ZIM-domain transcription factors, which are important negative regulators of the JA pathway. Comparatively, genes involved in the ET pathway were less affected by a BPH infestation in RH. DNA sequence analysis revealed that most BPH infestation-inducible genes may be regulated by the genetic background in a trans-acting manner, instead of by their promoters. We profiled the analysis of the global gene expression in RH and TN1 under BPH infestation, together with changes in the SA and JA levels. SA plays a leading role in the resistance response of rice to BPH. Our results will aid in understanding the molecular basis of RH’s BPH resistance and facilitate the identification of new resistance-related genes for breeding BPH-resistant rice varieties. The online version of this article (doi:10.1186/s12870-017-1005-7) contains supplementary material, which is available to authorized users.