Identification of the brackish water clam Corbicula japonica (Japanese name, Yamato-shijimi) and pecification of the growing district by PCR-based analysis of mitochondrial DNA
Identification of the brackish water clam Corbicula japonica (Japanese name, Yamato-shijimi) and pecification of the growing district by PCR-based analysis of mitochondrial DNA
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通过基于 PCR 的线粒体 DNA 分析鉴定咸水蛤 Corbicula japonica（日本名 Yamato-shijimi）并确定生长区
DOI：
10.1080/15275922.2011.572951
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Takeshita H
中科院分区：
文献类型：
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作者：
Fujihara;J;Yasuda;T;Ueki M;Fujita Y;Nakamura M;Oshiumi C;Hosozawa T;Nabika T;Harada Y;Kobayashi T;Nakajima T;Takeshita H
在这项研究中，一种新的聚合酶链反应（PCR）为基础的基因分型方法，以确定在日本生长的三个Corbiculasspecies（Corbicula japonica，Corbicula sandai，Corbicula laena）的发展。采用等位基因特异性PCR方法，对C. japonicacaccan被鉴定并且与种C.桑代和C. laenain组织和各种加工食品。此外，C.桑代和C.以Nla Ⅲ为限制性内切酶，采用PCR-限制性片段长度多态性（RFLP）方法，此外，还进行了初步的实验，以确定C.从七个地区中进行了japonicagrows。PCR-RFLP方法的使用使得有可能鉴定C.日本产于小川原湖。本研究采用电泳基因分型方法，对C。日本种可以被识别，并且可以容易地和可重复地指定特定生长区的种。这种方法对于保护生物资源和维持生态系统是有用的。本研究代表了该领域的贡献，因为这里提出的策略可以应用于其他物种的法医鉴定，如其他双壳类和软体动物。
In this study, a novel polymerase chain reaction (PCR)-based genotyping method to identify threeCorbiculaspecies growing in Japan (Corbicula japonica, Corbicula sandai, andCorbicula laena)was developed. Using an allele-specific PCR method,C. japonicacould be identified and specifically distinguished from the speciesC. sandaiandC. laenain both tissues and various processed foods. In addition, the discrimination ofC. sandaiandC. laenawas possible using the PCR-restriction fragment length polymorphism (RFLP) method withNlaIII as a restriction enzyme. Moreover, a preliminary experiment to determine the district in whichC. japonicagrows from among seven areas was performed. The use of the PCR-RFLP method made it possible to identifyC. japonicafrom Lake Ogawara. By using electrophoretic genotyping methods in the present study,C. japonicaspecies could be identified and the species of a particular growing district could be specified easily and reproducibly. This method is useful for protecting bioresources and maintaining ecosystems. The present study represents a contribution to the field because the strategy presented here can be applied to the forensic identification of other species, such as other bivalve and Mollusca.