Catechin Inhibits the Release of Advanced Glycation End Products during Glycated Bovine Serum Albumin Digestion and Corresponding Mechanisms In Vitro
Catechin Inhibits the Release of Advanced Glycation End Products during Glycated Bovine Serum Albumin Digestion and Corresponding Mechanisms In Vitro
复制标题
儿茶素抑制糖化牛血清白蛋白消化过程中高级糖化终产物的释放及体外相应机制
DOI：
10.1021/acs.jafc.1c03348
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发表时间：
2021
影响因子：
6.1
通讯作者：
Zhang Liang
中科院分区：
文献类型：
--
作者：
Wu Qian;Ouyang Yu;Kong Yingfei;Min Yaoyao;Xiao Juan;Li Shuyi;Zhou Mengzhou;Feng Nianjie;Zhang Liang
糖化蛋白是膳食高级糖基化终末产物(AGEs)的主要来源。糖化蛋白在胃肠道中被酶解，释放出更多可吸收的和更小的潜在有害AGE。研究了儿茶素对糖化牛血清白蛋白(G-BSA)在胃肠道消化过程中AGE释放的抑制作用。儿茶素抑制胃肠道消化过程中AGE的释放，尤其是在胃消化阶段。此外，儿茶素通过降低G-BSA的消化率改变了小肠中的这些多肽。其作用机制包括儿茶素与G-BSA/消化酶的相互作用、抑制消化酶活性和改变G-BSA的构象。儿茶素减少G-BSAβ-SHAP含量，保护螺旋构象。此外，儿茶素可增强G-BSA的抗氧化能力，从而减轻AGEs释放引起的餐后胃肠道氧化应激。这项研究加深了我们对儿茶素在胃肠道消化过程中对饮食年龄的营养和健康影响的理解。
Glycated proteins are the main source of dietary advanced glycation end products (AGEs). Glycated proteins are enzymatically hydrolyzed in the gastrointestinal tract, which releases more absorbable and smaller potentially harmful AGEs. This study investigated the inhibitory effect of catechin on AGE release from glycated bovine serum albumin (G-BSA) during gastrointestinal digestion. Catechin inhibited AGE release during gastrointestinal digestion, especially in the gastric digestion stage. Additionally, catechin altered these peptides in the small intestine by reducing G-BSA digestibility. The proposed mechanism involves interactions between catechin and G-BSA/digestive enzymes, inhibiting digestive enzyme activity and changing the conformation of G-BSA. Catechin reduced G-BSA β-sheet content and protected the helical conformation. Moreover, catechin enhanced the antioxidant capacity of G-BSA, which could attenuate postprandial oxidative stress in the gastrointestinal tract caused by the release of AGEs. This study improves our understanding of the nutritional and health effects of catechin on dietary AGEs during gastrointestinal digestion.