Filament arrangements in negatively stained cultured cells: the organization of actin.
Filament arrangements in negatively stained cultured cells: the organization of actin.
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负染培养细胞中的丝状排列：肌动蛋白的组织。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
J. Celis
中科院分区：
文献类型：
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作者：
J. Small;J. Celis
用Triton X-100处理扩散的培养细胞，然后进行负染色，揭示了未提取的细胞内丝状元件的组织：肌动蛋白、微管和100埃细丝。本报告描述了肌动蛋白样丝在人皮肤成纤维细胞和小鼠3 T3细胞的组织。如早期研究所示，细胞质应力纤维被认为是由成束的共线肌动蛋白样丝。除了这些大的应力纤维，还观察到小得多的细丝束以及随机取向的细丝。发现一束直径为0.2微米至0.5微米的粗细丝界定了分布良好的静止细胞中最突出的凹细胞边缘。人皮肤成纤维细胞的前缘和皱褶边缘表现为一个宽的网，由对角取向的细丝相互连接的放射状网状结构，直径约0.1微米的细丝的线性束。这些束对应于前面描述的微刺，长度约为1.5微米，横向间隔1微米至3微米。3个T3细胞的前缘显示出类似的组织，但较少的辐射细纤维束。纤维束和网状结构中的纤维与平滑肌肌球蛋白亚片段1形成箭头状复合物，该复合物是单极的，并指向细胞的主体。研究结果进行了讨论有关的机制，非肌肉细胞的运动。
Treatment of spread, cultured cells with Triton X-100 followed by negative staining reveals the organization of the unextracted intracellular filamentous elements: actin, microtubules and the 100 angstrom filaments. The present report describes the organization of the actin-like filaments in human skin fibroblasts and mouse 3 T 3 cells. As shown in earlier studies, the cytoplasmic stress fibres were seen to be composed of bundles of colinear actin-like filaments. In addition to these large stress fibres much smaller bundles of thin filaments as well as randomly oriented thin filaments were also observed. A thick bundle of thin filaments, 0.2 microm to 0.5 microm in diameter, was found to delimit the concave cell edges most prominent in well-spread stationary cells. The leading edge and ruffled border of human skin fibroblasts appeared as a broad web, of meshwork of diagonally oriented thin filaments interconnecting radiating, linear bundles of thin filaments about 0.1 microm in diameter. These bundles corresponding to the microspikes described earlier ranged from about 1.5 microm in length and were separated by 1 microm to 3 microm laterally. The leading edge of 3 T 3 cells showed a similar organization but with fewer radiating thin filament bundles. Both the filaments in the bundles and in the meshwork formed arrowhead complexes with smooth muscle myosin subfragment - 1 which were unipolar and directed towards the main body of the cell. The findings are discussed in relation to the mechanisms of non-muscle cell motility.