Mycoplasma hyopneumoniae-derived lipid-associated membrane proteins induce apoptosis in porcine alveolar macrophage via increasing nitric oxide production, oxidative stress, and caspase-3 activation
Mycoplasma hyopneumoniae-derived lipid-associated membrane proteins induce apoptosis in porcine alveolar macrophage via increasing nitric oxide production, oxidative stress, and caspase-3 activation
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猪肺炎支原体衍生的脂质相关膜蛋白通过增加一氧化氮产生、氧化应激和 caspase-3 激活诱导猪肺泡巨噬细胞凋亡
DOI：
10.1016/j.vetimm.2013.07.004
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发表时间：
2013-09-15
影响因子：
1.8
通讯作者：
Shao, Guoqing
中科院分区：
文献类型：
--
作者：
Bai, Fangfang;Ni, Bo;Shao, Guoqing
猪肺炎支原体是猪地方性肺炎的主要病原。支原体脂相关膜蛋白（LAMP）是支原体疾病的主要致病因子。本实验研究猪肺炎支原体LAMP对猪肺泡巨噬细胞（PAM） 3D4/21细胞系的影响。在lamp处理的PAM 3D4/21细胞中观察到染色质凝集和凋亡小体等凋亡特征。此外，与未处理的对照组相比，LAMP显著增加了PAM 3D4/21细胞中TUNEL阳性凋亡细胞的数量。此外，annexin-V-FITC和碘化丙啶双染色的流式细胞术分析显示，肺炎支原体LAMP诱导PAM 3D4/21细胞发生时间依赖性凋亡。此外，暴露于LAMP后，PAM 3D4/21细胞的超氧阴离子产生水平和活化的caspase-3水平均有所增加。细胞上清液中也证实了一氧化氮（NO）的产生增加。此外，NOS抑制剂或抗氧化剂可使凋亡率升高，抑制caspase-3的活化。提示肺炎支原体LAMP通过产生NO、产生超氧阴离子和激活caspase-3诱导猪肺泡巨噬细胞凋亡。(C) 2013 Elsevier B.V.版权所有
Mycoplasma hyopneumoniae is the primary etiological agent of enzootic pneumonia in swine. Lipid-associated membrane proteins (LAMP) of mycoplasma are the main pathogenicity factors in mycoplasma diseases. In this study, we investigated the effects of M. hyopneumoniae LAMP on porcine alveolar macrophage (PAM) 3D4/21 cell line. Apoptotic features, such as chromatin condensation and apoptotic bodies, were observed in LAMP-treated PAM 3D4/21 cells. Moreover, LAMP significantly increased the number of TUNEL positive apoptotic cells in PAM 3D4/21 cells compared with the untreated control. In addition, flow cytometric analysis using dual staining with annexin-V-FITC and propidium iodide (PI) showed that LAMP of M. hyopneumoniae induced a time-dependent apoptosis in PAM 3D4/21 cells. Moreover, increased levels of superoxide anion production and activated caspase-3 in PAM 3D4/21 cells were observed after exposure to LAMP. Increased production of nitric oxide (NO) was also confirmed in the cell supernatants. Besides, apoptotic rates increase and caspase-3 activation were suppressed by NOS inhibitor or antioxidant. It is suggested that LAMP of M. hyopneumoniae induced apoptosis in porcine alveolar macrophage via NO production, superoxide anion production, and caspase-3 activation. (C) 2013 Elsevier B.V. All rights reserved.