Covalent bonding of homochiral metal-organic framework in capillaries for stereoisomer separation by capillary electrochromatography
Covalent bonding of homochiral metal-organic framework in capillaries for stereoisomer separation by capillary electrochromatography
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DOI：
10.1002/elps.201500342
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发表时间：
2016-02-01
期刊：
影响因子：
2.9
通讯作者：
Li, Jian
中科院分区：
文献类型：
--
作者：
Ma, Jichao;Ye, Nengsheng;Li, Jian
本工作合成了一种[Cu（mal）（bpy）]中心点H2O（mal，L-（-）-malic acid; bpy，4，4 '-bipyridine）纯手性金属有机骨架（MOFs），并利用酰胺键将MOFs颗粒与毛细管内壁形成共价键连接，用于修饰毛细管内壁。采用X射线衍射、热重分析、粒度分布分析、氮吸附表征、红外光谱、扫描电镜和X射线能谱等对合成的[Cu（mal）（bpy）]中心点H2O和MOFs修饰毛细管柱进行了表征。用MOFs修饰的毛细管柱对某些药物的立体异构体进行了分离。6种分析物的检出限和定量限分别为0.1和0.25 μ g/mL。线性范围：麻黄碱0.25250 μ g/mL，伪麻黄碱0.25-250 μ g/mL，D-青霉胺0.25-180 μ g/mL，L-青霉胺0.25-120 μ g/mL，D-苯丙氨酸0.25-180 μ g/mL，L-苯丙氨酸0.25-160 μ g/mL，R-2均> 0.999。最后，将该修饰毛细管柱应用于真实的中药饮片中有效成分的分析。
In this work, a [Cu(mal)(bpy)] center dot H2O (mal, L-(-)-malic acid; bpy, 4,4 '-bipyridyl) homochiral metal-organic frameworks (MOFs) was synthesized and used for modifying the inner walls of capillary columns by utilizing amido bonds to form covalent links between the MOFs particles and capillary inner wall. The synthesized [Cu(mal)(bpy)] center dot H2O and MOFs-modified capillary column were characterized by X-ray diffraction, thermogravimetric analysis, particle size distribution analysis, nitrogen absorption characterization, FTIR spectroscopy, SEM, and energy-dispersive X-ray spectroscopy (EDX). The MOFs-modified capillary column was used for the stereoisomer separation of some drugs. The LODs and LOQs of six analytes were 0.1 and 0.25 mu g/mL, respectively. The linear range was 0.25250 mu g/mL for ephedrine, 0.25-250 mu g/mL for pseudoephedrine, 0.25-180 mu g/mL for Dpenicillamine, 0.25-120 mu g/mL for L-penicillamine, 0.25-180 mu g/mL for D-phenylalanine, and 0.25-160 mu g/mL for L-phenylalanine, all with R-2 > 0.999. Finally, the MOFs-modified capillary column was applied for the analysis of active ingredients in a real sample of the traditional Chinese medicine ephedra.