Elevated plasma α-defensins in patients with acute exacerbation of fibrotic interstitial pneumonia
Elevated plasma α-defensins in patients with acute exacerbation of fibrotic interstitial pneumonia
复制标题
DOI：
10.1016/j.rmed.2014.12.015
复制
发表时间：
2015-02-01
影响因子：
4.3
通讯作者：
Kohno, Shigeru
中科院分区：
文献类型：
--
作者：
Sakamoto, Noriho;Ishimatsu, Yuji;Kohno, Shigeru
背景：纤维化间质性肺疾病患者可发展为急性加重(AE)。AE的病因尚不清楚，但中性粒细胞可能在发病机制中起关键作用。中性粒细胞储存含有防御素的天青颗粒，防御素是抗菌肽，也在调节炎症反应中发挥作用。本研究通过对间质性肺炎(AE-IP)患者血浆防御素水平的检测，探讨防御素在AE-IP发病机制中的作用(S)，以及防御素能否作为AE-IP的生物标志物。方法：采用双抗体夹心ELISA法测定21例AE-IP、44例稳定(S)IP、9例IP合并肺部感染(InFEC-IP)患者血浆防御素水平。结果：S-IP组血浆HNP水平明显高于S-IP组，但与Infec-IP组差异无统计学意义，且与其他临床特征及预后无关。血浆HNP在鉴别AE-IP和S-IP方面无特异性。免疫组织化学分析显示，AE-IP患者积累的中性粒细胞中HNPs表达增加。血浆HBD2水平在AE-IP、S-IP和INFEC-IP之间无差异。结论：血浆HNPs水平在AE-IP组高于S-IP组，但还不足以作为AE-IP的候选生物标志物。防御素在AE-IP发病机制中的作用尚需进一步研究。(C)2015爱思唯尔有限公司。保留所有权利。
Background: Patients with fibrosing interstitial lung diseases can develop acute exacerbation (AE). The aetiology of AE remains obscure, but neutrophils might play a pivotal role in the pathogenesis. Neutrophils store azurophil granules containing defensins that are antimicrobial peptides that also function in regulating the inflammatory response. The present study evaluates plasma levels of defensins in patients with AE of interstitial pneumonia (AE-IP) to determine their role(s) in the pathogenesis of AE-IP and whether defensins could serve as a biomarker of AE-IP.Methods: Plasma levels of defensins including human neutrophil peptides (HNPs) and human beta defensin 2 (HBD2) were measured using ELISA in 21 patients with AE-IP, 44 with stable (S)-IP, nine with IP complicated with pulmonary infection (Infec-IP), and in 23 healthy volunteers. Lung HNP expression was immunohistochemically analyzed in biopsy and autopsy tissues diagnosed as S-IP and AE-IP.Results: Plasma levels of HNPs were significantly higher in patients with AE-IP than with S-IP, but did not differ from those with Infec-IP and were not associated with other clinical features and prognosis. Plasma HNP were not specific in terms of distinguishing AE-IP from S-IP. Imnnunohistochemical analysis showed increased HNPs expression in accumulated neutrophils from patients with AE-IP. Plasma levels of HBD2 did not differ among patients with AE-IP, S-IP and I nfec-IP.Conclusions: Elevated plasma levels of HNPs were higher in AE-IP than in S-IP, but not specific enough to serve as candidate biomarkers of AE-IP. Further studies are needed to clarify the role of defensins in the pathogenesis of AE-IP. (C) 2015 Elsevier Ltd. All rights reserved.