Comparison of premodulated interferential and pulsed current electrical stimulation in prevention of deep muscle atrophy in rats
Comparison of premodulated interferential and pulsed current electrical stimulation in prevention of deep muscle atrophy in rats
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DOI：
10.1007/s10735-012-9473-4
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发表时间：
2013-04
影响因子：
3.2
通讯作者：
Minoru Tanaka;Yusuke Hirayama;N. Fujita;H. Fujino
中科院分区：
文献类型：
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作者：
Minoru Tanaka;Yusuke Hirayama;N. Fujita;H. Fujino
本研究的目的是比较使用脉冲电流 (PC) 和预调制干扰电流 (IC) 的电刺激对预防小腿深层肌肉层肌肉萎缩的效果。将大鼠随机分为 3 个治疗组：对照组、后肢卸载 2 周 (HU) 和 HU 加电刺激 2 周。电刺激组的动物在卸载期间每天接受两次左小腿肌肉（PC）或右小腿肌肉（IC）的治疗性刺激。与对照动物相比，接受 HU 两周的动物表现出肌肉质量显着损失、肌纤维横截面积 (CSA) 减少、腓肠肌和比目鱼肌中泛素化蛋白表达增加。 PC 刺激减弱了对腓肠肌中肌肉质量、纤维 CSA 和泛素化蛋白的影响。然而，PC刺激未能阻止腓肠肌和比目鱼肌深层的萎缩。相反，IC 刺激可抑制腓肠肌和比目鱼肌的萎缩。此外，IC 方案抑制了 HU 诱导的腓肠肌和比目鱼肌中泛素化蛋白表达的增加。这些结果表明，IC 电刺激在预防四肢肌肉深层肌肉萎缩方面比 PC 更有效。
The goal of this study was to compare the effects of electrical stimulation using pulsed current (PC) and premodulated interferential current (IC) on prevention of muscle atrophy in the deep muscle layer of the calf. Rats were randomly divided into 3 treatment groups: control, hindlimb unloading for 2 weeks (HU), and HU plus electrical stimulation for 2 weeks. The animals in the electrical stimulation group received therapeutic stimulation of the left (PC) or right (IC) calf muscles twice a day during the unloading period. Animals undergoing HU for 2 weeks exhibited significant loss of muscle mass, decreased cross-sectional area (CSA) of muscle fibers, and increased expression of ubiquitinated proteins in the gastrocnemius and soleus muscles compared with control animals. Stimulation with PC attenuated the effects on the muscle mass, fiber CSA, and ubiquitinated proteins in the gastrocnemius muscle. However, PC stimulation failed to prevent atrophy of the deep layer of the gastrocnemius muscle and the soleus muscle. In contrast, stimulation with IC inhibited atrophy of both the gastrocnemius and soleus muscles. In addition, the IC protocol inhibited the HU-induced increase in ubiquitinated protein expression in both gastrocnemius and soleus muscles. These results suggest that electrical stimulation with IC is more effective than PC in preventing muscle atrophy in the deep layer of limb muscles.