Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe
Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe
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DOI：
10.21769/bioprotoc.3128
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发表时间：
2019-01-05
期刊：
影响因子：
0.8
通讯作者：
Cucullo, Luca
中科院分区：
文献类型：
--
作者：
Sivandzade, Farzane;Bhalerao, Aditya;Cucullo, Luca
近年来，荧光染料已被频繁地用于监测线粒体膜电位，以评估线粒体的活力和功能。然而，实验室间结果的重现性在很大程度上取决于以下经过充分验证和可靠的方案沿着适当的控制。在这里，我们提供了一个实用的用户指南，用于监测线粒体膜电位在整个细胞使用荧光阳离子探针。我们提供的线粒体膜电位的数据分析与外源性物质（如吸烟）对血脑屏障内皮细胞（包括小鼠原代（mBMEC）和基于小鼠的内皮细胞系（bEnd3））的影响相关，并进行了并排比较。
In recent years, fluorescent dyes have been frequently used for monitoring mitochondrial membrane potential to evaluate mitochondrial viability and function. However, the reproducibility of the results across laboratories strongly depends upon following well validated and reliable protocols along with the appropriate controls. Herein, we provide a practical user guide for monitoring mitochondrial membrane potential in whole cells using a fluorescent cationic probe. The data analysis of mitochondrial membrane potential we provide is one associated with the impact of xenobiotics such as tobacco smoking on blood-brain barrier endothelial cells including both mouse primary (mBMEC) and a mouse-based endothelial cell line (bEnd3) in a side by side comparison.