Estrogen receptor measurement in low-protein breast cancer cytosols. A modified charcoal technique.
Estrogen receptor measurement in low-protein breast cancer cytosols. A modified charcoal technique.
复制标题
低蛋白乳腺癌细胞质中雌激素受体的测量。
DOI:
10.1007/bf01807889
复制
发表时间:
1981
影响因子:
3.8
通讯作者:
McGuire,WL
中科院分区:
文献类型:
--
作者:
Powell,BL;DeLaGarza,M;Clark,GM;McGuire,WL
The assay of estrogen receptor (ER) in human breast cancer biopsies is usually excellent using the dextrancoated charcoal method. If the cytosol protein concentration is below 1 mg/ml, however, underestimation of ER content occurs presumably due to adsorption of ER to the charcoal. This problem can be minimized if 1 mg/ml of bovine serum albumin (BSA) is added to the charcoal solution prior to use.