Estrogen receptor measurement in low-protein breast cancer cytosols. A modified charcoal technique.

Estrogen receptor measurement in low-protein breast cancer cytosols. A modified charcoal technique.
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低蛋白乳腺癌细胞质中雌激素受体的测量。

DOI:
10.1007/bf01807889
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发表时间:
1981
影响因子:
3.8
通讯作者:
McGuire,WL
McGuire,WL
中科院分区:
医学2区
文献类型:
--
作者:
Powell,BL;DeLaGarza,M;Clark,GM;McGuire,WL

文献摘要

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用葡聚糖包被活性炭法测定乳腺癌活检标本中的雌激素受体(ER)通常是很好的。然而,如果胞质溶胶蛋白浓度低于1 mg/ml,则可能由于ER吸附到活性炭上而发生ER含量的低估。如果在使用前向活性炭溶液中加入1 mg/ml牛血清白蛋白(BSA),则可将此问题降至最低。
The assay of estrogen receptor (ER) in human breast cancer biopsies is usually excellent using the dextrancoated charcoal method. If the cytosol protein concentration is below 1 mg/ml, however, underestimation of ER content occurs presumably due to adsorption of ER to the charcoal. This problem can be minimized if 1 mg/ml of bovine serum albumin (BSA) is added to the charcoal solution prior to use.