An omega protein from Drosophila melanogaster.
An omega protein from Drosophila melanogaster.
复制标题
来自黑腹果蝇的欧米伽蛋白。
DOI：
10.1021/bi00718a009
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发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
J. Wang
中科院分区：
文献类型：
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作者：
W. Baase;J. Wang
Walter A. Baase和James C.王 * 摘要：从果蝇卵中部分纯化出一种蛋白质，它能将超螺旋DNA转化为不含超螺旋转角的共价闭合形式。该活性类似于大肠杆菌的蛋白质（Wang，JC（1971），J. Mol. Biol. Biol.55，523），其中：（1）在DNA中除了超螺旋转角的损失之外没有检测到变化;（2）超螺旋转角的损失不遵循单次命中机制，并且随着反应的进行，可以获得具有减少的超螺旋转角数目的DNA中间体;（3）没有daily-^最近，来自大肠杆菌的一种蛋白质已经被证明可以从负超螺旋DNA中去除超螺旋转角，而不会在两条链中的任何一条上留下单链断裂。（Wang，1971，1973）。共价闭合双链的拓扑约束
Walter A. Baase and James C. Wang* abstract: A protein which converts superhelical DNA to a covalently closed form containing no superhelical turns has been partially purified from the eggs of Drosophila melanogaster. This activity is similar to the protein of Escherichia coli (Wang, JC (1971), J. Mol. Biol. 55, 523) in that:(1) no change except the loss of superhelical turns can be detected in the DNA;(2) the loss of superheli-cal turns does not follow a single-hit mechanism, and DNA intermediates with a decreasing number of superhelical turns can be obtained as the reaction proceeds;(3) no dialy-^ Recently, a protein from Escherichia coli has been shown to remove superhelical turns from a negative su-perhelical DNA without leaving a single chain scission in either one of the two strands (Wang, 1971, 1973). The to-pological constraint for a covalently closed double-stranded