Effects of downregulating TEAD4 transcripts by RNA interference on early development of bovine embryos.
Effects of downregulating TEAD4 transcripts by RNA interference on early development of bovine embryos.
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DOI：
10.1262/jrd.2016-130
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发表时间：
2017-04-21
期刊：
影响因子：
--
通讯作者：
Sawai K
中科院分区：
文献类型：
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作者：
Sakurai N;Takahashi K;Emura N;Hashizume T;Sawai K
转录因子TEA结构域家族转录因子4（Tead 4）是参与小鼠胚胎滋养外胚层（TE）分化的关键因子之一。然而，目前关于TEAD 4在牛胚胎着床前发育中的作用的知识是有限的。本研究通过RNA干扰技术检测了TEAD 4的转录本和蛋白表达模式，并试图阐明TEAD 4在牛植入前发育过程中的功能。TEAD 4 mRNA被发现上调之间的16细胞和桑椹胚阶段，和TEAD 4蛋白的核定位检测桑椹胚阶段，以及在随后的发展阶段。TEAD 4下调直到囊胚期才影响胚胎发育，并且TEAD 4下调的胚胎在5%和21%O2条件下都能够形成TE。基因表达分析结果显示，TEAD 4下调不影响POU 5类转录因子1（OCT-4）、NANOG、尾型同源框2（CDX 2）、加塔结合蛋白3（GATA 3）和干扰素-tau（IFNT）的表达水平。因此，TEAD 4基因可能在牛胚胎的囊胚期发育和TE分化中发挥重要作用。
Transcription factor TEA domain family transcription factor 4 (Tead4) is one of the key factors involved in the differentiation of the trophectoderm (TE) in murine embryos. However, knowledge on the roles of TEAD4 in preimplantation development during bovine embryos is currently limited. This study examined the transcript and protein expression patterns of TEAD4 and attempted to elucidate the functions of TEAD4 during bovine preimplantation development using RNA interference. TEAD4 mRNA was found to be upregulated between the 16-cell and morula stages, and nuclear localization of the TEAD4 protein was detected at the morula stage, as well as in subsequent developmental stages. TEAD4 downregulation did not affect embryonic development until the blastocyst stage, and TEAD4-downregulated embryos were capable of forming the TE under both 5% and 21% O2 conditions. Results of gene expression analysis showed that TEAD4 downregulation did not affect the expression levels of POU class 5 transcription factor 1 (OCT-4), NANOG, caudal-type homeobox 2 (CDX2), GATA binding protein 3 (GATA3), and interferon-tau (IFNT). In conclusion, TEAD4 might be dispensable for development until the blastocyst stage and TE differentiation in bovine embryos.