Preparation of PCL/silk fibroin/collagen electrospun fiber for urethral reconstruction
Preparation of PCL/silk fibroin/collagen electrospun fiber for urethral reconstruction
复制标题
尿道重建用PCL/丝素/胶原电纺纤维的制备
DOI：
10.1007/s11255-014-0854-3
复制
发表时间：
2015-01-01
影响因子：
2
通讯作者：
Li, Hongbin
中科院分区：
文献类型：
--
作者：
Wei, Gaijie;Li, Chao;Li, Hongbin
为制备聚己内酯（PCL）/丝素蛋白/胶原蛋白电纺生物支架并观察其对口腔黏膜上皮细胞生长增殖的影响，将再生丝素蛋白膜、水溶性胶原蛋白粉和PCL按1：1：4、1：1：8和1：1：10的质量比溶解于六氟异丙醇中，采用静电纺丝法制备PCL/丝素蛋白/胶原蛋白电纺生物支架。将体外培养的口腔黏膜上皮细胞接种于材料表面，采用MTT比色法和扫描电镜观察口腔黏膜上皮细胞在材料表面的生长增殖情况，MTT法检测结果表明，口腔黏膜上皮细胞在PCL/丝素蛋白/胶原纤维上生长良好，胶原静电纺丝支架。扫描电镜观察表明，制备的静电纺纤维直径均匀，呈现相互连通的多孔网状结构，口腔黏膜上皮细胞在改性材料表面生长形态良好; PCL/丝素蛋白/胶原静电纺生物支架具有合适的孔径和孔隙率，适合口腔黏膜上皮细胞生长，具有良好的细胞相容性，是组织工程尿道重建的良好支架。
To prepare polycaprolactone (PCL)/silk fibroin/collagen electrospun nanofiber scaffold and test its effects on growth and proliferation of oral mucosal epithelial cells.Regenerated silk fibroin film, water-soluble collagen powder, and PCL, at mass ratios of 1:1:4, 1:1:8, and 1:1:10, were dissolved in hexafluoroisopropanol, and electrostatic spinning method was adopted to prepare PCL/silk fibroin/collagen electrospun nanofiber scaffold. In vitro cultured oral mucosal epithelial cells were inoculated on the material surface, MTT assay and scanning electron microscopy were adopted to study the growth and proliferation of oral mucosal epithelial cells on the material surface, and cell compatibility of PCL/silk fibroin/collagen electrospun nanofiber was evaluated.The result of MTT assay showed that oral mucosal epithelial cells were growing well on the PCL/silk fibroin/collagen electrospun nanofiber scaffold. Scanning electron microscopy showed that the prepared electrospun fiber was uniform in diameter and presented an interconnected porous net structure, and oral mucosal epithelial cells had a good growth form on the surface of the modified material.PCL/silk fibroin/collagen electrospun nanofiber scaffold has appropriate pore size and porosity, is suitable for the growth of oral mucosal epithelial cells, has good cell compatibility, and is a good scaffold for tissue engineering urethral reconstruction.