The Development of Collagen Nerve Conduits that Promote Peripheral Nerve Regeneration
The Development of Collagen Nerve Conduits that Promote Peripheral Nerve Regeneration
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促进周围神经再生的胶原神经导管的发展
DOI：
10.1007/978-1-4615-3844-8_23
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
R. Madison
中科院分区：
文献类型：
--
作者：
Shu;S. Archibald;C. Krarup;R. Madison
我们已经研究了周围神经的修复在动物模型中使用管状导管。用于制造神经导管的材料及其物理化学和机械特性可以影响再生的程度、速率和形态。导管膜的渗透性是似乎在神经再生中起重要作用的一个参数。在本研究中，两种类型的神经导管的牛肌腱胶原蛋白具有明显不同的渗透性。导管膜的渗透性通过各种大小的分子穿过这些膜的扩散来确定。一种类型的导管具有仅允许葡萄糖等小分子通过的孔（小孔胶原导管）。另一种类型具有易于渗透大分子如牛血清白蛋白的孔（大孔胶原导管）。当在小鼠中测试桥接坐骨神经的4mm间隙时，大孔胶原导管比小孔胶原导管更大程度地支持神经再生。对大鼠和灵长类动物的研究表明，大孔胶原导管在运动和感觉反应的生理恢复方面与神经自体移植物一样有效。这些管道的体外和体内研究结果代表了我们朝着开发合适的现成假体用于临床修复受损周围神经的特定目标迈出的重要一步。
We have investigated the repair of peripheral nerves in animal models using tubular guiding conduits. The materials used to fabricate the nerve conduits and their physicochemical and mechanical characteristics can influence the extent, rate and morphology of regeneration. Permeability of the conduit membranes is one parameter which seems to play an important role in nerve regeneration. In the present study, two types of nerve conduits were developed from bovine tendon collagen with distinctly different permeabilities. The permeability of the conduit membranes was determined by diffusion of various sized molecules across these membranes. One type of conduit had pores which only allowed small molecules such as glucose to pass (small pore collagen conduits). The other type had pores which were readily permeable to macromolecules such as bovine serum albumin (large pore collagen conduits). The large pore collagen conduits supported nerve regeneration to a greater degree than the small pore collagen conduits when tested in mice to bridge 4 mm gaps of the sciatic nerve. Studies in rats and primates suggested that large pore collagen conduits worked as effectively as nerve autografts in terms of physiological recovery of motor and sensory responses. The results ofin vitroandin vivostudies of these conduits represent a significant step towards our specific aim of developing suitable off-the-shelf prostheses for clinical repair of damaged peripheral nerves.