A mutation with major effects on Drosophila melanogaster sex pheromones
A mutation with major effects on Drosophila melanogaster sex pheromones
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DOI：
10.1534/genetics.104.033159
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发表时间：
2005-12-01
期刊：
影响因子：
3.3
通讯作者：
Ferveur, JF
中科院分区：
文献类型：
--
作者：
Marcillac, F;Bousquet, F;Ferveur, JF
性信息素是种内的化学信号，对配偶吸引和辨别至关重要。在黑腹果蝇中，成熟雌性和雄性果蝇角质层上的主要碳氢化合物截然不同，往往分别刺激或抑制雄性求爱。这种性别差异很大程度上取决于由去饱和酶添加的双键的数量（雄性一个，雌性两个）。突变是由插入desat 1基因的PGal 4转座子引起的，该基因编码对设置这些双键至关重要的去饱和酶。纯合子突变果蝇产生的性信息素比对照果蝇少70-90%，两性之间的信息素差异几乎消失。通过去除全部或部分转座子，共诱导出134个切除等位基因。信息素谱通常在具有完全或大部分去除的转座子的切除等位基因中被拯救，而在具有较大片段的转座子的等位基因中它保持突变。在幼虫到成虫发育过程中检测到5个desal 1转录本。它们的水平在24小时的成年人中被精确量化，这是性信息素产生的关键时期。三个成绩单显着不同的控制女性和男性之间，但是，占主导地位的成绩单显示没有差异。在突变果蝇中，主要的成绩单是高度下降的两个性二态性成绩单。这两个转录本在姊妹种D中也不存在。simulans，它没有显示性二态烃。我们还诱导了一个幼虫致死等位基因，缺乏所有的成绩单，并未能补充突变等位基因的缺陷烃表型。
Sex pheromones are intraspecific chemical signals that are crucial for mate attraction and discrimination. In Drosophila melanogaster the predominant hydrocarbons on the Cuticle of mature female and male flies are radically different and tend to stimulate or inhibit male Courtship, respectively. This Sexual difference depends largely upon the number of double bonds (one in males and two in females) added by desaturase enzymes. A mutation was caused by a PGal4 transposon inserted in the desat1 gene that codes for the desaturase crucial for setting these double bonds. Homozygous mutant flies produced 70-90% fewer sex pheromones than control flies, and the pheromonal difference between the sexes was almost abolished. A total of 134 excision alleles were induced by pulling out all or a part of the transposon. The pheromonal profile was generally rescued in excision alleles with a completely or largely removed transposon whereas it remained Mutant in alleles with a larger piece of the transposon. Five desal1 transcripts were detected during larval-to-adult development. Their levels were precisely quantified in 24-hr old adults, a critical period for the production of sex pheromones. Three transcripts significantly varied between control females and males; however, the predominant transcript showed no difference. In mutant flies, the predominant transcript was highly decreased with the two sexually dimorphic transcripts. These two transcripts were also absent in the sibling species D. simulans, which shows no sexually dimorphic hydrocarbons. We also induced a larval-lethal allele that lacked all transcripts and failed to complement the defective hydrocarbon phenotype of mutant alleles.