Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV
Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV
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DOI：
10.1007/bf02889755
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发表时间：
2004-04
影响因子：
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通讯作者：
N. Jin;Hongyong Zhang;Gefen Yin;M. Zheng;Tong Liu;Wenzheng Jiang;Zi-jian Li
中科院分区：
文献类型：
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作者：
N. Jin;Hongyong Zhang;Gefen Yin;M. Zheng;Tong Liu;Wenzheng Jiang;Zi-jian Li
将口蹄疫病毒结构蛋白前体P1-2A和蛋白酶3C分别插入鸡痘病毒重组载体pUTA-2和pUTA-16-LacZ中，构建了重组表达载体pUTA2P1和pUTAL3CP1，经RT-PCR、IFA和Western blotting鉴定，获得了两株重组FPVs(vUTA2P1和vUTAL3CP1)。分别注射rFPV的小鼠可诱导出高水平的抗口蹄疫病毒抗体，T细胞亚群增多，脾细胞杀伤活性高于对照组。本研究为口蹄疫病毒候选疫苗的构建提供了一种新的方法。
Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot- and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV.