Systematic identification and characterization of Aedes aegypti long noncoding RNAs (lncRNAs)
Systematic identification and characterization of Aedes aegypti long noncoding RNAs (lncRNAs)
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DOI：
10.1038/s41598-019-47506-9
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发表时间：
2019-08-21
影响因子：
4.6
通讯作者：
Azzarn, Ghows
中科院分区：
文献类型：
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作者：
Azlan, Azali;Obeidat, Sattam M.;Azzarn, Ghows
长链非编码rna （lncRNAs）在生物过程中发挥着多种作用。埃及伊蚊（伊蚊）埃及伊蚊是一种吸血蚊子，是负责复制和传播虫媒病毒（包括登革热、寨卡病毒和基孔肯雅病毒）的主要媒介。伊蚊的系统鉴定和发育特征。埃及的lncrna仍然有限。我们进行了lncRNA的全基因组鉴定，随后进行了Ae中lncRNA的发育分析。蚊。我们共鉴定出4,689个新的lncRNA转录本，其中2,064个，2,076个和549个分别是基因间的，内含子的和反义的。Ae。埃及伊蚊的lncrna与其他物种具有低表达、低GC含量、短长度和低保守性等特点。此外，Ae。埃及伊蚊lncRNAs倾向于与邻近的反义蛋白编码基因相关。lncrna的一个子集显示了母系遗传的证据；因此，提示lncrna在早期胚胎中的潜在作用。此外，lncrna更倾向于以发育特异性和时间特异性的方式表达。本研究结果为进一步研究Ae的功能奠定了基础。蚊lncRNAs。
Long noncoding RNAs (lncRNAs) play diverse roles in biological processes. Aedes aegypti (Ae. aegypti), a blood-sucking mosquito, is the principal vector responsible for replication and transmission of arboviruses including dengue, Zika, and Chikungunya virus. Systematic identification and developmental characterisation of Ae. aegypti lncRNAs are still limited. We performed genome-wide identification of lncRNAs, followed by developmental profiling of lncRNA in Ae. aegypti. We identified a total of 4,689 novel lncRNA transcripts, of which 2,064, 2,076, and 549 were intergenic, intronic, and antisense respectively. Ae. aegypti lncRNAs share many characteristics with other species including low expression, low GC content, short in length, and low conservation. Besides, the expression of Ae. aegypti lncRNAs tend to be correlated with neighbouring and antisense protein-coding genes. A subset of lncRNAs shows evidence of maternal inheritance; hence, suggesting potential role of lncRNAs in early-stage embryos. Additionally, lncRNAs show higher tendency to be expressed in developmental and temporal specific manner. The results from this study provide foundation for future investigation on the function of Ae. aegypti lncRNAs.