Constitutional abnormality of nuclear membrane proteins in small cell lung carcinoma
Constitutional abnormality of nuclear membrane proteins in small cell lung carcinoma
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DOI：
10.1007/s00428-019-02597-7
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发表时间：
2019-10-01
期刊：
影响因子：
3.5
通讯作者：
Katoh, Ryohei
中科院分区：
文献类型：
--
作者：
Wang, Jieying;Kondo, Tetsuo;Katoh, Ryohei
据报道，核膜蛋白在维持核结构和协调细胞活动中起重要作用。研究核膜蛋白的谱可以帮助我们评估恶性肿瘤的生物学和/或临床性质。使用emerin、lamin A/C、lamin B和LAP 2抗体的免疫组织化学，我们检查了来自33个小细胞肺癌（SCLC）和72个非SCLC（34个腺癌、30个鳞状细胞癌和8个大细胞癌）的105个肺癌组织。Emerin在79%的SCLC和1%的非SCLC中呈阴性或局部/弱阳性，核纤层蛋白A/C在91%的SCLC和3%的非SCLC中呈类似阳性。LAP 2在SCLC和非SCLC中的表达相似。RT-PCR分析表明，在SCLC细胞系中，Emerin和Lamin A/C的mRNA表达明显下调，支持免疫组化结果。总之，我们认为，核膜蛋白Emerin和核纤层蛋白A/C的下调是小细胞肺癌细胞的特征，这种核膜的结构异常可能与小细胞肺癌的生物学和/或临床性质有关。此外，了解小细胞肺癌细胞的核蛋白谱可能有助于我们理解肺活检标本中被称为压碎伪影的核脆性。
Nuclear membrane proteins reportedly play important roles in maintaining nuclear structures and coordinating cell activities. Studying profiles of nuclear membrane proteins may help us evaluate the biological and/or clinical nature of malignant tumors. Using immunohistochemistry with antibodies for emerin, lamin A/C, lamin B, and LAP2, we examined 105 lung cancer tissues from 33 small cell lung carcinomas (SCLCs) and 72 non-SCLCs (34 adenocarcinomas, 30 squamous cell carcinomas, and 8 large cell carcinomas). Emerin had negative or local/weak positivity in 79% of SCLCs and 1% of non-SCLCs, and lamin A/C had similar positivity in 91% of SCLCs and 3% of non-SCLCs. LAP2's expression was similar between SCLCs and non-SCLCs. RT-PCR analyses for these four nuclear membrane proteins over 7 cell lines showed that mRNA of emerin and lamin A/C were distinctly downregulated in the SCLC cell lines, supporting the immunohistochemical results. In conclusion, we suggest that downregulation of the nuclear membrane proteins emerin and lamin A/C is characteristic of SCLC cells, and this constitutional abnormality of the nuclear membrane may be related to the biological and/or clinical nature of SCLC. In addition, knowing the nuclear protein profile in SCLC cells may contribute to our understanding of nuclear fragility known as the crush artifact in pulmonary biopsy specimens.