CLONING AND EXPRESSION OF 2 BRAIN-SPECIFIC INWARDLY RECTIFYING POTASSIUM CHANNELS
CLONING AND EXPRESSION OF 2 BRAIN-SPECIFIC INWARDLY RECTIFYING POTASSIUM CHANNELS
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DOI：
10.1073/pnas.92.15.6753
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发表时间：
1995-07-18
影响因子：
11.1
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
--
作者：
BREDT, DS;WANG, TL;SNYDER, SH
我们克隆了两个内向整流钾离子通道，它们选择性地发生在脑神经元中，并被命名为比尔克（脑内向整流钾离子）通道。BIRK 1 mRNA非常丰富，并在特定的脑干核中富集，BIRK 1显示出一个共有的磷酸结合环，在非洲爪蟾卵母细胞中的表达表明其电导被ATP和腺苷5 '-[γ-硫代]三磷酸抑制。BIRK 2的丰度要低得多，并且选择性地定位于端脑神经元中。BIRK 2具有cAMP依赖性磷酸化的共有序列。
We have cloned two inwardly rectifying K+ channels that occur selectively in neurons in the brain and are designated BIRK (brain inwardly rectifying K+) channels. BIRK1 mRNA is extremely abundant and is enriched in specific brainstem nuclei, BIRK1 displays a consensus phosphate-binding loop, and expression in Xenopus oocytes has shown that its conductance is inhibited by ATP and adenosine 5'-[gamma-thio] triphosphate. BIRK2 is far less abundant and is selectively localized in telencephalic neurons. BIRK2 has a consensus sequence for cAMP-dependent phosphorylation.