Plasmodium falciparum susceptibility to standard and potential anti-malarial drugs in Dakar, Senegal, during the 2013-2014 malaria season
Plasmodium falciparum susceptibility to standard and potential anti-malarial drugs in Dakar, Senegal, during the 2013-2014 malaria season
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DOI：
10.1186/s12936-015-0589-3
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发表时间：
2015-02-06
期刊：
影响因子：
3
通讯作者：
Pradines, Bruno
中科院分区：
文献类型：
--
作者：
Fall, Becaye;Camara, Cheikhou;Pradines, Bruno
背景资料：2006年，塞内加尔国家疟疾防治方案建议将青蒿素类复方疗法作为无并发症疟疾的一线治疗。自从采用ACT以来，关于恶性疟原虫对抗疟药物的耐药性水平的报告很少。对从达喀尔医院获得的当地分离株进行了离体敏感性研究（达喀尔，塞内加尔）进行。本研究测定了18株恶性疟原虫对氯喹（CQ）、奎宁（QN）、阿莫地喹的活性代谢物单去乙基阿莫地喹（MDAQ）、甲氟喹（MQ）、本芴醇（LMF）、青蒿琥酯（AS）、结果：敏感性降低的菌株对MQ、CQ、QN和MDAQ的检出率分别为55.6%、50%、5.6%，而对DHA、AS和LMF的检出率为0%。PND，PPQ和PVB的平均IC 50分别为5.8 nM，32.2 nM和5.3 nM，respectively.Conclusions：在达喀尔，MQ敏感性降低的分离株的流行率仍然很高，而且稳定。自2004年以来，CQ耐药的流行率有所下降，但在2013年在达喀尔反弹。PND、PPQ和PVB在达喀尔的恶性疟原虫中显示出较高的体外活性。
Background: In 2006, the Senegalese National Malaria Control Programme recommended artemisinin-based combination therapy (ACT) as the first-line treatment for uncomplicated malaria. Since the introduction of ACT, there have been very few reports on the level of Plasmodium falciparum resistance to anti-malarial drugs. An ex vivo susceptibility study was conducted on local isolates obtained from the Hopital Principal de Dakar (Dakar, Senegal) from November 2013 to January 2014.Methods: Eighteen P. falciparum isolates were sussessfully assessed for ex vivo susceptibility to chloroquine (CQ), quinine (QN), monodesethylamodiaquine (MDAQ), the active metabolite of amodiaquine, mefloquine (MQ), lumefantrine (LMF), artesunate (AS), dihydroartemisinin (DHA), the active metabolite of artemisinin derivatives, pyronaridine (PND), piperaquine (PPQ), and, Proveblue (PVB), a methylene blue preparation, using the HRP2-based ELISA test.Results: The prevalence of isolates with reduced susceptibility was 55.6% for MQ, 50% for CQ, 5.6% for QN and MDAQ, and 0% for DHA, AS and LMF. The mean IC50 for PND, PPQ and PVB were 5.8 nM, 32.2 nM and 5.3 nM, respectively.Conclusions: The prevalence of isolates with a reduced susceptibility to MQ remains high and stable in Dakar. Since 2004, the prevalence of CQ resistance decreased, but rebounded in 2013 in Dakar. PND, PPQ and PVB showed high in vitro activity in P. falciparum parasites from Dakar.