Rapid Embedding in Lowicryl K4M for Immunoelectron Microscopic Studies
Rapid Embedding in Lowicryl K4M for Immunoelectron Microscopic Studies
复制标题
快速嵌入 Lowicryl K4M 中进行免疫电子显微镜研究
DOI：
10.1002/jemt.1060060402
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
E. Carlemalm
中科院分区：
文献类型：
--
作者：
G. Simon;Jennifer A Thomas;K. Chorneyko;E. Carlemalm
建立了一种快速包埋Lowicryl K4M组织的方法。用苯甲酸乙酯代替引发剂，将间接紫外光下的聚合温度由- 35℃提高到- 10℃。在24小时内发生完全聚合。与原方法和Altman等人提出的其他两种快速方法进行比较。细胞化学，32:1217-1223,1984)，上述方法重复性好，组织保存效果好。对大鼠外分泌胰腺酶原颗粒的抗淀粉酶和对金仓鼠卵的抗透明带进行了抗原性测试，结果表明抗原性保存良好。比较使用新的快速方法保存抗原性的结果表明，与原来的Lowicryl K4M和Epon相比，使用快速Lowicryl K4M方法增加了特异性标记。原始方法和快速方法之间的背景水平没有显著差异。然而，与Epon相比，两种Lowicryl K4M方法存在显著差异。这种快速方法的可靠性使其不仅适合于研究，而且适合作为常规的超微结构水平诊断组织化学技术。
A rapid method of embedding tissues in Lowicryl K4M was developed. The initiator was replaced with benzoin ethyl ether, and the temperature of polymerization under indirect ultraviolet light was increased from −35°C to −10°C. The complete polymerization occurred in 24 hours. Compared with the original method and two other rapid methods proposed by Altman et al. (J. Histochem. Cytochem. 32:1217–1223, 1984), the above mentioned rapid method gave more reproducible results, and the preservation of the tissues was better. The preservation of the antigenicity was excellent as tested with anti-amylase on zymogen granules of the rat's exocrine pancreas and with anti-zona pellucida on ova of the golden hamster. Comparison of the preservation of antigenicity by means of the new rapid method shows an increased specific labeling when using the rapid Lowicryl K4M method as compared to the original Lowicryl K4M and Epon. Background levels between the original and rapid Lowicryl K4M methods are not significantly different. However, the two Lowicryl K4M methods differ significantly when compared to Epon. The reliability of this rapid method makes it suitable for introduction not only for research but also as a routine diagnostic histochemical technique at the ultrastructural level.
DOI：
--
发表时间：
1982
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
M. Bendayan;J. Reddy
通讯作者：
M. Bendayan;J. Reddy