High-Resolution Melting Analysis for accurate detection of BRAF mutations: a systematic review and metaanalysis
High-Resolution Melting Analysis for accurate detection of BRAF mutations: a systematic review and metaanalysis
复制标题
DOI：
10.1038/srep04168
复制
发表时间：
2014-02-25
影响因子：
4.6
通讯作者：
Fu, Wei-Ling
中科院分区：
文献类型：
--
作者：
Chen, Dong;Wang, Yan-Yan;Fu, Wei-Ling
高分辨率熔解曲线分析（HRMA）可能是一种快速检测BRAF突变的良好方法。然而，HRMA检测BRAF突变的准确性尚未得到系统评价。我们进行了系统回顾和荟萃分析，涉及14个独立研究的1324个样本。HRMA的总体灵敏度为0.99（95%置信区间（CI）= 0.75-0.82），总体特异性非常高，为0.99（95% CI = 0.94-0.98）。合并阳性似然比、阴性似然比和诊断比值比的值分别为68.01（95% CI = 25.33-182.64）、0.06（95% CI = 0.03-0.11）和1263.76（95% CI = 393.91-4064.39）。相同数据的汇总受试者工作特征曲线显示面积为1.00，Q* 值为0.97。HRMA具有灵敏度高、特异性强、操作简便、成本低、省时省力、周转快等优点，是临床上快速检测BRAF基因突变的一种较好的替代方法。
The high-resolution melting curve analysis (HRMA) might be a good alternative method for rapid detection of BRAF mutations. However, the accuracy of HRMA in detection of BRAF mutations has not been systematically evaluated. We performed a systematic review and meta-analysis involving 1324 samples from 14 separate studies. The overall sensitivity of HRMA was 0.99 (95% confidence interval (CI) 5 0.75-0.82), and the overall specificity was very high at 0.99 (95% CI = 0.94-0.98). The values for the pooled positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio were 68.01 (95% CI = 25.33-182.64), 0.06 (95% CI = 0.03-0.11), and 1263.76 (95% CI = 393.91-4064.39), respectively. The summary receiver operating characteristic curve for the same data shows an area of 1.00 and a Q* value of 0.97. The high sensitivity and specificity, simplicity, low cost, less labor or time and rapid turnaround make HRMA a good alternative method for rapid detection of BRAF mutations in the clinical practice.