Expression of ATP sensor protein in Caenorhabditis elegans
Expression of ATP sensor protein in Caenorhabditis elegans
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ATP传感器蛋白在秀丽隐杆线虫中的表达
DOI：
10.1002/jemt.21103
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
and K. Yokoyama
中科院分区：
文献类型：
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作者：
J. Kishikawa;M. Fujikawa;H. Imamura;K. Yasuda;H. Noji;N. Ishii;S. Mitani;and K. Yokoyama
5′-三磷酸腺苷 (ATP) 是主要的能量货币，参与许多生物过程。动物细胞的 ATP 监测系统有助于研究能量代谢与生物过程之间的关系。据报道，荧光 ATP 生物传感器 ATeam（基于 Epsilon 亚基的 ATP 指示剂，用于分析测量）可基于荧光共振能量转移 (FRET) 监测培养细胞中的 ATP 水平，通过显微注射和紫外线照射方法将其引入线虫中。为了确认 ATeam 是否在线虫细胞中充当 ATP 传感器，作者测量了转基因线虫细胞中 ATeam 的 FRET。 ATeam 在线虫的靶细胞中表达。在外阴细胞中，细胞质中的 ATP 水平高于线粒体中的 ATP 水平。 ATeam 还检测到了转基因线虫培养细胞中 ATP 水平的变化。这些实验表明 ATeam 可用于检测线虫细胞中 ATP 水平的变化。显微镜。资源。技术，2012 年。© 2011 Wiley periodicals, Inc.
Adenosine 5′‐triphosphate (ATP) is the major energy currency and is involved in many biological processes. The ATP‐monitoring system for cells in animals can be helpful to study the relationship between energy metabolism and biological processes. The fluorescent ATP biosensor ATeam (ATP indicator based on Epsilon subunit for Analytical Measurements), which has been reported to monitor ATP levels in cultured cells on the basis of fluorescence resonance energy transfer (FRET), was introduced into nematodes by microinjection and UV‐irradiation method. To confirm whether ATeam functions as an ATP sensor in nematode cells, the authors measured FRET of ATeam in cells of transgenic nematode. The ATeam was expressed in target cells in nematode. In vulva cells, ATP levels in the cytosol were higher than those in mitochondria. ATeam also sensed ATP level change in cultured cells from the transgenic nematode. These experiments indicated that ATeam is available for detection of changes in ATP levels in nematode cells. Microsc. Res. Tech., 2012. © 2011 Wiley Periodicals, Inc.