Effects of various sera on growth kinetics and collagen synthesis by keloid and normal dermal fibroblasts.
Effects of various sera on growth kinetics and collagen synthesis by keloid and normal dermal fibroblasts.
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各种血清对疤痕疙瘩和正常真皮成纤维细胞生长动力学和胶原合成的影响。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
Cohen Ik
中科院分区：
文献类型：
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作者：
McCoy Bj;Cohen Ik
：源自正常真皮和疤痕疙瘩的成纤维细胞在补充有胎牛血清、疤痕疙瘩患者血清以及年龄、性别和种族匹配的非疤痕疙瘩形成者血清的营养培养基中培养。在生长期的不同时间测定细胞数量，并在第7天分析胶原合成。在滞后期，与正常真皮成纤维细胞相比，所有血清中瘢痕疙瘩来源的成纤维细胞均显着减少。然而，第 2 天之后，两种细胞类型在疤痕疙瘩和对照人血清中的生长动力学相似。此外，在疤痕疙瘩和对照人血清中，胶原蛋白合成相对于总蛋白质合成（百分比）是相似的。尽管全身因素与疤痕疙瘩和其他纤维化疾病（例如硬皮病和肺纤维化）的发病机制有关，但本研究表明，疤痕疙瘩患者的血清不含有显着改变疤痕疙瘩来源或正常真皮成纤维细胞的体外生长动力学或胶原合成的因素。
: Fibroblasts derived from normal dermis and keloids were cultured in nutrient medium supplemented with fetal bovine serum, serum from keloid patients, and serum from age-, sex-, and race-matched nonkeloid formers. Cell numbers were determined at various times during the growth period, and collagen synthesis was analyzed on day 7. In lag phase, keloid-derived fibroblasts were significantly reduced in all sera compared with normal dermal fibroblasts. However, beyond day 2, the growth kinetics of both cell types were similar in keloid and control human sera. Furthermore, collagen synthesis relative to total protein synthesis (percent) was similar in keloid and control human sera. Although systemic factors have been implicated in the pathogenesis of keloids and other fibrotic diseases such as scleroderma and pulmonary fibrosis, this study demonstrates that serum from keloid patients does not contain a factor(s) that significantly modifies the in vitro growth kinetics or collagen synthesis of keloid-derived or normal dermal fibroblasts.