3-DIMENSIONAL TERTIARY STRUCTURE OF YEAST PHENYLALANINE TRANSFER-RNA
3-DIMENSIONAL TERTIARY STRUCTURE OF YEAST PHENYLALANINE TRANSFER-RNA
复制标题
DOI：
10.1126/science.185.4149.435
复制
发表时间：
1974-01-01
期刊：
影响因子：
56.9
通讯作者：
RICH, A
中科院分区：
文献类型：
--
作者：
KIM, SH;SUDDATH, FL;RICH, A
结晶酵母苯丙氨酸转移RNA的3埃电子密度图为我们提供了完整的三维模型，该模型定义了分子中所有核苷酸残基的位置。该分子的整体特征实际上与在 4 埃分辨率下看到的特征相同，只是三级结构的许多附加细节现在已可视化。确定了十种类型的氢键，它们定义了三级相互作用的特异性。该分子还通过平面嘌呤和嘧啶的大量堆积而稳定。这种三级结构以简单直接的方式解释了转移RNA的化学修饰研究。由于大多数三级相互作用涉及所有转移RNA共有的核苷酸，因此这种三维结构很可能提供了基本的折叠模式，这可能有助于阐明所有转移RNA的三维结构。
The 3-angstrom electron density map of crystalline yeast phenylalanine transfer RNA has provided us with a complete three-dimensional model which defines the positions of all of the nucleotide residues in the molecule. The overall features of the molecule are virtually the same as those seen at a resolution of 4 angstroms except that many additional details of tertiary structure are now visualized. Ten types of hydrogen bonding are identified which define the specificity of tertiary interactions. The molecule is also stabilized by considerable stacking of the planar purines and pyrimidines. This tertiary structure explains, in a simple and direct fashion, chemical modification studies of transfer RNA. Since most of the tertiary interactions involve nucleotides which are common to all transfer RNA's, it is likely that this three-dimensional structure provides a basic pattern of folding which may help to clarify the three-dimensional structure of all transfer RNA's.