Correction to: Increasing of activity and thermostability of cold active butanol-tolerant endoglucanase from a marine Rhodococcus sp. under high concentrations of butanol condition.
Correction to: Increasing of activity and thermostability of cold active butanol-tolerant endoglucanase from a marine Rhodococcus sp. under high concentrations of butanol condition.
复制标题
修正：海洋红球菌属耐冷丁醇内切葡聚糖酶的活性和热稳定性增加。
DOI：
10.1007/978-3-540-85156-1_331
复制
发表时间：
2018
期刊：
影响因子：
2.8
通讯作者：
Zeng X
中科院分区：
文献类型：
--
作者：
Zeng X
由于标准标本制备技术的局限性，使用透射电子显微镜（TEM）对细胞和生物材料之间的界面进行成像和化学制图经常被证明是一个有趣的挑战。在过去，超显微切开术主要用于组织切片，然而较硬的材料，如骨和植入生物材料之间的界面也被切片。这为材料硬度接近的材料的分析提供了一种有效且普遍可靠的方法。然而，将软细胞附着在硬材料上的超薄样品的制备可能会在样品中引入切割人工制品。此外，超微切开术需要在切割前对细胞进行化学固定、脱水并嵌入树脂中，耗费时间和资源。最近，低温聚焦离子束扫描电子显微镜（cryo- fisem）与TEM低温转移相结合已成为制备此类样品的替代途径。
The imaging and chemical mapping of the interface between cells and biomaterials using transmission electron microscopy (TEM) has often proved to be an interesting a challenge, due to the limitations of standard specimen preparation techniques. In the past, ultramicrotomy has mainly been used to section tissue, however harder materials such as the interface between bone and implanted biomaterials have also been sectioned [1]. This has provided an effective and generally reliable methodology for the analysis of materials which are close in material hardness, However, the preparation of ultrathin samples of soft cells attached to hard materials may introduce cutting artefacts into the specimen. Also, ultramicrotomy requires cells to be chemically fixed, dehydrated and embedded in resin prior to cutting, consuming time and resources. Recently, cryo focused ion beam scanning electron microscopy (cryo-FIBSEM) coupled with cryo transfer for TEM has emerged as an alternative pathway to ultramicrotomy for the preparation of such samples.