Requirement for the PDZ domain protein, INAD, for localization of the TRP store-operated channel to a signaling complex
Requirement for the PDZ domain protein, INAD, for localization of the TRP store-operated channel to a signaling complex
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DOI：
10.1016/s0896-6273(01)80049-0
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发表时间：
1997-01-01
期刊：
影响因子：
16.2
通讯作者：
Montell, C
中科院分区：
文献类型：
--
作者：
Chevesich, J;Kreuz, AJ;Montell, C
在果蝇中，光感受器细胞需要钙库操纵的钙通道（TRP）来维持对光的反应。在这里，我们表明，TRP与磷脂酶C-β（NORPA），视紫红质（RH 1），钙调蛋白，和PDZ域含有蛋白质INAD形成复合物。具有PDZ结构域的蛋白质先前已显示在体外聚集离子通道。我们表明，在InaD突变苍蝇，TRP不再是空间限制其正常的亚细胞区室，横纹肌。这些结果提供了证据，PDZ结构域蛋白是必需的，在体内，锚定的离子通道的信号复合物。此外，这种相互作用的破坏导致视网膜变性。我们建议TRP通道连接到NORPA和RH 1，以促进这些上游信号分子的反馈调节。
In Drosophila, the store-operated Ca2+ channel, TRP, is required in photoreceptor cells for a sustained response to light. Here, we show that TRP forms a complex with phospholipase C-beta (NORPA), rhodopsin (RH1), calmodulin, and the PDZ domain containing protein INAD. Proteins with PDZ domains have previously been shown to cluster ion channels in vitro. We show that in InaD mutant flies, TRP is no longer spatially restricted to its normal subcellular compartment, the rhabdomere. These results provide evidence that a PDZ domain protein is required, in vivo, for anchoring of an ion channel to a signaling complex. Furthermore, disruption of this interaction results in retinal degeneration. We propose that the TRP channel is linked to NORPA and RH1 to facilitate feedback regulation of these upstream signaling molecules.