Prolonged wakefulness alters neuronal responsiveness to local electrical stimulation of the neocortex in awake rats..
Prolonged wakefulness alters neuronal responsiveness to local electrical stimulation of the neocortex in awake rats..
复制标题
长时间的清醒会改变清醒大鼠神经元对新皮质局部电刺激的反应。
DOI：
10.1111/jsr.12009
复制
发表时间：
2013
影响因子：
4.4
通讯作者：
Tononi,Giulio
中科院分区：
文献类型：
--
作者：
Vyazovskiy,VladyslavV;Olcese,Umberto;Cirelli,Chiara;Tononi,Giulio
长时间的清醒或睡眠不足会导致认知缺陷，但对潜在的细胞机制知之甚少。我们最近发现，睡眠剥夺会影响睡眠和清醒大鼠新皮层的自发神经元活动。虽然众所周知，突触反应是由正在进行的皮层活动调制，它仍然不清楚是否延长清醒影响皮层神经元对传入刺激的反应。通过应用局部电微刺激的额叶区的新皮层，我们发现，经过4小时的清醒期后，在对侧额叶皮层的初始神经元反应更强，更同步，其次是一个更深刻的抑制神经元尖峰相比，控制条件。诱发活动的这些变化表明神经元兴奋性增加，并表明，在保持清醒后，皮层神经元变得短暂兴奋。我们认为，睡眠剥夺的一些不利影响可能是神经元对传入的内在和外在输入的反应性改变的结果。
Prolonged wakefulness or a lack of sleep lead to cognitive deficits, but little is known about the underlying cellular mechanisms. We recently found that sleep deprivation affects spontaneous neuronal activity in the neocortex of sleeping and awake rats. While it is well known that synaptic responses are modulated by ongoing cortical activity, it remains unclear whether prolonged waking affects responsiveness of cortical neurons to incoming stimuli. By applying local electrical microstimulation to the frontal area of the neocortex, we found that after a 4 h period of waking the initial neuronal response in the contralateral frontal cortex was stronger and more synchronous, and was followed by a more profound inhibition of neuronal spiking as compared with the control condition. These changes in evoked activity suggest increased neuronal excitability and indicate that, after staying awake, cortical neurons become transiently bistable. We propose that some of the detrimental effects of sleep deprivation may be a result of altered neuronal responsiveness to incoming intrinsic and extrinsic inputs.