STEREOCHEMISTRY OF REACTIONS CATALYZED BY MAMMALIAN-BRAIN L-GLUTAMATE 1-CARBOXY-LYASE AND 4-AMINOBUTYRATE - 2-OXOGLUTARATE AMINOTRANSFERASE
STEREOCHEMISTRY OF REACTIONS CATALYZED BY MAMMALIAN-BRAIN L-GLUTAMATE 1-CARBOXY-LYASE AND 4-AMINOBUTYRATE - 2-OXOGLUTARATE AMINOTRANSFERASE
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DOI：
10.1111/j.1432-1033.1979.tb13195.x
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
LIPPERT, B
中科院分区：
文献类型：
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作者：
BOUCLIER, M;JUNG, MJ;LIPPERT, B
哺乳动物或细菌的 4-氨基丁酸转氨酶对 4-氨基丁酸的脱氨基作用涉及 4-氨基丁酸 C-4 上原 S 氢的提取。大鼠脑谷氨酸脱羧酶对 L-谷氨酸进行脱羧，并保留构型。 (S)-4-氨基己-5-炔酸对该酶的抑制涉及抑制剂 C-4 处质子的夺取。谷氨酸脱羧酶存在异常反应，其中(S)-4-氨基己-5-炔酸的C-4处的质子以类似于L-氨基酸的酶促氨基转移中通常发生的1的方式被去除。该反应可能是由与消除的质子相邻的炔基促进的。
Deamination of 4-aminobutyrate by mammalian or bacterial 4-aminobutyrate aminotransferases involves the abstraction of the pro-S hydrogen on C-4 of 4-aminobutyrate. Decarboxylation of L-glutamate by rat brain glutamate decarboxylase occurs with retention of configuration. Inhibition of this enzyme by (S)-4-aminohex-5-ynoic acid involves the abstraction of the proton at C-4 of the inhibitor. The existence of an abnormal reaction of glutamate decarboxylase in which the proton at C-4 of (S)-4-aminohex-5-ynoic acid is removed in a manner similar to the 1 which normally occurs in enzymatic transaminations of L-amino acids. This reaction is presumably facilitated by the acetylenic group adjacent to the eliminated proton.