Expression of proinflammatory, proatherogenic genes is reduced by the Mediterranean diet in elderly people
Expression of proinflammatory, proatherogenic genes is reduced by the Mediterranean diet in elderly people
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DOI：
10.1017/s0007114511005812
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发表时间：
2012-08-14
影响因子：
3.6
通讯作者：
Lopez-Miranda, Jose
中科院分区：
文献类型：
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作者：
Camargo, Antonio;Delgado-Lista, Javier;Lopez-Miranda, Jose
衰老是动脉粥样硬化发展速度的一个重要决定因素，主要是通过慢性低度炎症的产生。饮食，特别是其脂肪含量，调节空腹和餐后状态下的炎症反应。我们的目的是研究膳食脂肪对炎症相关基因表达的影响（NF-κ B、单核细胞趋化蛋白1（MCP-1）、TNF-α和IL-6）和斑块稳定性（基质金属蛋白酶9，MMP-9）在20名健康老年人的餐后状态下，他们遵循三种饮食，每种饮食3周：通过随机交叉设计，（1）富含MUFA和初榨橄榄油的地中海饮食（Med Diet）;（2）富含SFA的饮食;和（3）富含n-3 PUFA的低脂高碳水化合物饮食（CHO-PUFA饮食）。在每个阶段结束时，在禁食12小时后，受试者接受了与饮食阶段结束时相似的早餐。在禁食状态下，与富含SFA的饮食相比，中等饮食导致NF-kB p65亚基的基因表达较低（P=0.019）。与富含SFA的饮食相比，中等饮食的摄入诱导了较低的p65（P=0.033）、MCP-1（P=0.0229）和MMP-9（P=0.041）的基因餐后表达，并且与CHO-PUFA饮食相比，诱导了较低的p65（P=0.027）和TNF-α（P=0.047）的基因餐后表达。直接血浆定量主要再现了这些结果。我们的数据表明，在老年人中，与富含SFA和CHO-PUFA的饮食相比，中等饮食的消费减少了单核细胞的餐后炎症反应。这些发现可能是部分负责较低的心血管疾病的风险，发现在人群中具有较高的坚持中的饮食。
Ageing is an important determinant of atherosclerosis development rate, mainly by the creation of a chronic low-grade inflammation. Diet, and particularly its fat content, modulates the inflammatory response in the fasting and postprandial states. Our aim was to study the effects of dietary fat on the expression of genes related to inflammation (NF-kappa B, monocyte chemoattractant protein 1 (MCP-1), TNF-alpha and IL-6) and plaque stability (matrix metalloproteinase 9, MMP-9) during the postprandial state of twenty healthy, elderly people who followed three diets for 3 weeks each: (1) Mediterranean diet (Med Diet) enriched in MUFA with virgin olive oil; (2) SFA-rich diet; and (3) lowfat, high-carbohydrate diet enriched in n-3 PUFA (CHO-PUFA diet) by a randomised crossover design. At the end of each period, after a 12-h fast, the subjects received a breakfast with a composition similar to the one when the dietary period ended. In the fasting state, the Med Diet consumption induced a lower gene expression of the p65 subunit of NF-kB compared with the SFA-rich diet (P=0.019). The ingestion of the Med Diet induced a lower gene postprandial expression of p65 (P=0.033), MCP-1 (P=0.0229) and MMP-9 (P=0.041) compared with the SFA-rich diet, and a lower gene postprandial expression of p65 (P=0.027) and TNF-alpha (P=0.047) compared with the CHO-PUFA diet. Direct plasma quantification mostly reproduced the findings. Our data suggest that consumption of a Med Diet reduces the postprandial inflammatory response in mononuclear cells compared with the SFA-rich and CHO-PUFA diets in elderly people. These findings may be partly responsible for the lower CVD risk found in populations with a high adherence to the Med Diet.