High frequency DNA rearrangement at qgamma27 creates a novel allele for Quality Protein Maize breeding.
High frequency DNA rearrangement at qgamma27 creates a novel allele for Quality Protein Maize breeding.
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qgamma27 处的高频 DNA 重排为优质蛋白玉米育种创造了一个新颖的等位基因。
DOI：
10.1038/s42003-019-0711-0
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Wu Yongrui
中科院分区：
文献类型：
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作者：
Liu Hongjun;Huang Yongcai;Li Xiaohan;Wang Haihai;Ding Yahui;Kang Congbin;Sun Mingfei;Li Fangyuan;Wang Jiechen;Deng Yiting;Yang Xuerong;Huang Xing;Gao Xiaoyan;Yuan Lingling;An Dong;Wang Wenqin;Holding David R;Wu Yongrui
拷贝数变异（CNV）是遗传变异的主要来源，通常会导致玉米表型变异。 27-kDa γ-玉米醇溶蛋白位点 (qγ27) 的重复对于将优质蛋白玉米 (QPM) 中的软胚乳转化为硬胚乳至关重要。这种重复是不稳定的，通常在该位点产生 CNV。我们进行了遗传实验，旨在直接测量不同遗传背景的男性和女性中发生的 DNA 重排频率。重复序列重新排列为单个拷贝的平均频率为 1.27 × 10−3，并且在不同的品系中有所不同。筛选出γ27基因三倍体，在未来QPM育种中表现出比重复体更好的潜力。我们的结果强调了一种直接确定 DNA 重排频率的新方法，在这种情况下会在 qγ27 位点产生 CNV。此外，这提供了一种在 QPM 育种中测试合适亲本的高效方法。
Copy number variation (CNV) is a major source of genetic variation and often contributes to phenotypic variation in maize. The duplication at the27-kDa γ-zeinlocus (qγ27) is essential to convert soft endosperm into hard endosperm in quality protein maize (QPM). This duplication is unstable and generally produces CNV at this locus. We conducted genetic experiments designed to directly measure DNA rearrangement frequencies occurring in males and females of different genetic backgrounds. The average frequency with which the duplication rearranges to single copies is 1.27 × 10−3and varies among different lines. A triplication ofγ27gene was screened and showed a better potential than the duplication for the future QPM breeding. Our results highlight a novel approach to directly determine the frequency of DNA rearrangements, in this case resulting in CNV at theqγ27locus. Furthermore, this provides a highly effective way to test suitable parents in QPM breeding.