Centrifugation-augmented solid-phase immunoassay (CASPIA) for the rapid diagnosis of infectious diseases.
Centrifugation-augmented solid-phase immunoassay (CASPIA) for the rapid diagnosis of infectious diseases.
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离心增强固相免疫分析 (CASPIA) 用于快速诊断传染病。
DOI：
10.1093/infdis/154.2.301
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Yolken,RH
中科院分区：
文献类型：
--
作者：
Miotti,PG;Viscidi,RP;Eiden,J;Cerny,E;Yolken,RH
我们设计了离心增强固相免疫分析（CASPIA）来检测人体体液中的微生物病原体。 CASPIA 形式结合了乳胶凝集和固相免疫分析系统的多种特征，产生了一种廉价且易于执行的分析系统。我们发现用于检测轮状病毒、A 组链球菌和 b 型流感嗜血杆菌抗原的 CASPIA 系统明显比乳胶凝集测定更敏感，并且至少与使用类似试剂的酶免疫测定系统一样敏感。由于 CASPIA 反应可以宏观读取、使用标准办公室复印设备记录或通过微孔板比色计定量，因此该测定适用于广泛的临床和实验室条件。
We have devised centrifugation-augmented solid-phase immunoassays (CASPIAs) for the detection of microbial pathogens in human body fluids. The CASPIA format combines several features of latex agglutination and solid-phase immunoassay systems to produce an assay system that is inexpensive and simple to perform. We found that CASPIA systems for the detection of rotaviral, group A streptococcal, andHaemophilus influenzaetype b antigens were significantly more sensitive than latex agglutination assays and at least as sensitive as enzyme immunoassay systems using analogous reagents. Because CASPIA reactions could either be read macroscopically, recorded with a standard office photocopying device, or quantitated by means of a microplate colorimeter, the assays were applicable under a wide range of clinical and laboratory conditions.