Ultrasensitive colorimetric detection of Salmonella enterica Typhimurium on lettuce leaves by HRPzyme-Integrated polymerase chain reaction
Ultrasensitive colorimetric detection of Salmonella enterica Typhimurium on lettuce leaves by HRPzyme-Integrated polymerase chain reaction
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DOI：
10.1016/j.foodcont.2017.09.010
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发表时间：
2018-02-01
期刊：
影响因子：
6
通讯作者：
Kim, Min-Gon
中科院分区：
文献类型：
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作者：
Kim, Seong U.;Batule, Bhagwan S.;Kim, Min-Gon
肠道沙门氏菌是最常见的食源性疾病病原体之一。这些疾病的爆发通常与食用新鲜水果和蔬菜有关。在这项研究中，我们报告了一个简单的比色检测策略的S。基于聚合酶链反应（PCR）产物，对鲜切莴苣叶中的肠道血清型鼠伤寒沙门氏菌进行检测，所述PCR产物由整合有辣根过氧化物酶模拟DNA酶（HRPzyme）的基因特异性引物产生。将HRPzyme序列整合在S.鼠伤寒杆菌在PCR反应结束时，未扩增的HRP酶整合的引物在氯化血红素的存在下折叠成G-四链体结构，然后它们用H2 O2催化2，2 '-连氮双（3-乙基苯并噻唑啉磺酸）（ABTS）的氧化。氧化ABTS比色信号强度与S.肠伤寒浓度。后一种关系证明了这种快速、简单、高灵敏度和选择性的比色平台对S.肠伤寒检测。(C)2017爱思唯尔有限公司版权所有
Salmonella enterica is one of the most encountered causative pathogens of food-borne illnesses. Outbreaks of such diseases are commonly associated with the consumption of fresh fruits and vegetables. In this study, we report a simple colorimetric strategy for the detection of S. enterica Serovar Typhimurium in fresh-cut lettuce leaves based on polymerase chain reaction (PCR) products generated by gene-specific primers integrated with the horseradish peroxidase-mimicking DNAzyme (HRPzyme). The HRPzyme sequence was integrated at the 5' end of the forward and reverse primers specific to 16S rRNA of S. enterica Typhimurium. At the end of the PCR reaction, unamplified HRPzyme-integrated primers were folded into G-quadruplex structure in the presence of hemin and then, they catalyzed the oxidation of 2,2'-azinobis (3-ethylbenzothiazolinesulfonic acid) (ABTS) with H2O2. The intensity of oxidized ABTS colorimetric signal was linearly and inversely related to S. enterica Typhimurium concentration. The latter relationship demonstrated diagnostic potential of this rapid, simple, highly sensitive, and selective colorimetric platform for S. enterica Typhimurium detection. (C) 2017 Elsevier Ltd. All rights reserved.