Quantifying mixing using equilibrium reactions
Quantifying mixing using equilibrium reactions
复制标题
使用平衡反应量化混合
DOI：
10.1063/1.3078247
复制
发表时间：
2009
影响因子：
4.6
通讯作者：
J. Posner
中科院分区：
文献类型：
--
作者：
P. Wheat;J. Posner
提出了一种利用氟-4和钙离子的荧光反应来量化微通道中的平衡反应的方法。在适当的条件下，平衡反应可以用来量化流体混合，而不存在与组分混合措施相关的挑战，如有限的成像空间分辨率和视角与三维结构耦合。在Y形微通道中测量了CaCl2和指示钙的荧光染料Fluo-4混合的定量测量。反应物和产物的浓度分布用格林函数解和对流扩散方程的数值解来模拟。当反应物浓度大于其解离常数的100倍且扩散系数相等时，平衡反应提供了一种明确的、定量的混合度量。在较低浓度和不同扩散系数下，在物种间相互作用之前，面平均荧光信号达到最大值。
A method of quantifying equilibrium reactions in a microchannel using a fluorometric reaction of Fluo-4 and Ca2+ ions is presented. Under the proper conditions, equilibrium reactions can be used to quantify fluid mixing without the challenges associated with constituent mixing measures such as limited imaging spatial resolution and viewing angle coupled with three-dimensional structure. Quantitative measurements of CaCl and calcium-indicating fluorescent dye Fluo-4 mixing are measured in Y-shaped microchannels. Reactant and product concentration distributions are modeled using Green’s function solutions and a numerical solution to the advection-diffusion equation. Equilibrium reactions provide for an unambiguous, quantitative measure of mixing when the reactant concentrations are greater than 100 times their dissociation constant and the diffusivities are equal. At lower concentrations and for dissimilar diffusivities, the area averaged fluorescence signal reaches a maximum before the species have interdi...
影响因子：
16.6
作者：
Song, H;Tice, JD;Ismagilov, RF
通讯作者：
Ismagilov, RF
DOI：
10.1016/s0021-9258(18)83165-9
发表时间：
1989-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Minta;J. Kao;R. Tsien
通讯作者：
A. Minta;J. Kao;R. Tsien
DOI：
10.1016/0006-291x(91)91966-g
发表时间：
1991-05-31
影响因子：
3.1
作者：
LATTANZIO, FA;BARTSCHAT, DK
通讯作者：
BARTSCHAT, DK