B-CELL DIFFERENTIATION IN THE CBA-N MOUSE .1. SLOWER MATURATION OF MITOGEN AND ANTIGEN-RESPONSIVE B-CELLS IN MICE EXPRESSING AN X-LINKED DEFECT
B-CELL DIFFERENTIATION IN THE CBA-N MOUSE .1. SLOWER MATURATION OF MITOGEN AND ANTIGEN-RESPONSIVE B-CELLS IN MICE EXPRESSING AN X-LINKED DEFECT
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DOI：
10.1084/jem.150.6.1483
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发表时间：
1979-01-01
影响因子：
15.3
通讯作者：
WATSON, JD
中科院分区：
文献类型：
--
作者：
WHITLOCK, CA;WATSON, JD
年龄对B [骨髓衍生]细胞的促有丝分裂和抗原反应性的影响在CBA/N和（CBA/N × N）的脾细胞培养物中进行了检测。DBA/2）F1小鼠。与年龄匹配的雌性F1小鼠相比，来自年轻雄性F1小鼠（4-6周龄）的脾细胞对脂多糖[LPS]的促有丝分裂反应较低，绵羊红细胞（SRBC）反应性B细胞前体的频率较低，携带IG[免疫球蛋白]细胞的百分比较低。随着F1代雄性小鼠年龄的增长，所有3种功能的表达均增加。而在60周龄的F1小鼠表现出相同百分比的免疫球蛋白承载脾细胞和类似的有丝分裂反应的LPS相比，成年雌性F1小鼠，SRBC反应性B细胞前体的频率仍然低3倍。在表达X连锁缺陷的小鼠中，B细胞的成熟较慢，并且该缺陷显然对B细胞的抗原和有丝分裂原活化机制具有不同的影响。
The effect of age on the mitogenic and antigenic responsiveness of B [bone marrow-derived] cells was examined in spleen cell cultures of CBA/N and (CBA/N .times. DBA/2) F1 mice. Spleen cells from young male F1 mice (4-6 wk old) show lower mitogenic responses to lipopolysaccharide [LPS], a lower frequency of sheep erythrocytes (SRBC)-reactive B cell precursors and a lower percentage of Ig[immunoglobulin]-bearing cells than age-matched female F1 mice. The expression of all 3 functions increased with age of the F1 male mice. Whereas F1 mice at 60 wk of age showed an equivalent percentage of Ig-bearing spleen cells and a similar mitogenic responsiveness to LPS when compared to adult female F1 mice, frequency of SRBC-reactive B cell precursors remained 3-fold lower. There is slower maturation of B cells in mice expressing the X-linked defect and the defect apparently has differential effects on the mechanisms of antigen and mitogen activation of B cells.